摘要
目的检测BAT2L蛋白在大鼠脑内的发育学表达情况。方法分别采用Western blot、免疫组化、免疫荧光技术检测BAT2L蛋白在胚胎18d,出生1、7、15d,成年不同时期大鼠脑内的表达、分布、定位情况。结果①Western blot结果显示,BAT2L在胚胎18d,出生1、7d大鼠脑内有高水平表达,而出生15d和成年则表达水平较低。②免疫组化、免疫荧光检测其在大鼠脑内的分布、定位情况:结果显示脑内阳性着色部位为神经元的细胞膜、细胞质,而细胞核、细胞外间质不着色;阳性神经元广泛分布于各个时期的大鼠大脑皮质、小脑皮质、海马、丘脑等部位;阳性神经元的形态在新生阶段和成年大鼠有明显差异,新生大鼠脑组织中部分神经元的突起及分支呈强阳性着色,而成年大鼠神经元突起几乎不着色。结论BAT2L蛋白特异性表达于大鼠大脑的神经元胞体、胞膜及突起中。
Objective To detect the expression of BAT2L protein in developmental brain of rats.Methods Expression,distribution and location of BAT2L protein in developmental brain rats on embryonic day 18,postnatal days 1,7 and 15 and in adult at different time points were detected by Western blotting analysis,immunohistochemistry and immunofluorescence,respectively.Results Western blotting showed that the BAT2L protein expression level was higher in brain of rats on embryonic day 18,postnatal days 1 and 7 than on postnatal day 15 and in brain of adult rats.Immunohistochemistry and immunofluorescence showed that the BAT2L protein was distributed and located in cell membrane and cytoplasm of neurons but not in nuclei and extra-cellular space.Positive neurons were widely distributed in cerebral and cerebellar cortex,hippocampus and cerebral ganglion.The morphology of neurons was significantly different in newborn and adult rats.Positive prominences and branches of certain neurons were found in brain tissues of newborn rats but hardly in those of adult rats.Conclusion BAT2L protein is specifically expressed in cell body,membrane and prominence of neurons in brain of rats.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第9期922-925,共4页
Journal of Third Military Medical University
基金
重庆市自然科学基金(CSTC2007BB5048)~~
关键词
中枢神经系统
发育
轴突生长
central nervous system
development
axon growth
