摘要
目的检测细胞表面抗原CD123(IL-3受体α链)在急性白血病(AL)患者骨髓干祖细胞中的表达,并探讨其与患者预后的关系。方法采用流式细胞术检测43例初治的AL患者及13例正常对照组的骨髓CD34阳性细胞中CDl23的表达情况;同时根据AL患者的年龄、细胞遗传学改变、发病时外周血白细胞数量将其按不同预后分为高危、标危、低危3组,其中高危15例,标危15例,低危13例,分析CD123表达与AL患者预后的相关性。结果43例AL患者CD34+CDl23+/CD34+平均水平为38.24%,显著高于对照组平均表达水平1.90%;3组AL患者CD34+CDl23+/CD34+均显著高于对照组(P<0.05);高危组患者CD34+CDl23+/CD34+显著高于标危组和低危组,差异具有统计学意义(P<0.05);标危组患者CD34+CDl23+/CD34+显著高于低危组,差异具有统计学意义(P<0.05)。结论在AL患者骨髓干祖细胞中,CD123异常过度表达;在高危组、标危组、低危组的表达逐渐降低,提示CD123的表达与AL患者的预后相关,临床可通过检测该指标判断AL患者预后。
Objective To investigate the expression of cell surface antigen CD123 on bone marrow stem cells and progenitor cells of the patients with acute leukemia（AL）and its relationship with prognosis.Methods By the means of flow cytometry,the expression of cell surface antigen CD123 on CD34＋cells of 43 initial treatment AL patients and 13 normal controls were detected respectively;At the same time,these AL patients were divided into 3 groups of different prognosis according to age,change of cell genetics and PWBC quantity of patients,15 of high risk group,15 of standard risk group and 13 of low risk group;Then the correlation between expression of CD123 and prognosis of AL patients were analyzed.Results CD34＋CDl23＋/CD34＋of 3 groups of AL patients were higher than those of control group（P〈0.05）;CD34＋CDl23＋/CD34＋of high risk group were significantly higher than standard risk group and low risk group,the difference has statistical significance（P〈0.05）;CD34＋CDl23＋/CD34＋of standard risk group were significantly higher than those of low risk group,the difference also has statistical significance（P〈0.05）.Conclusion The cell surface antigen CD123 is expressed excessively on bone marrow stem cells and progenitor cells of the patients with acute leukemia;The expression decreased gradually from the high risk group to the low risk group,it hints that the expression of CD123 is correlated with prognosis;it might be taken as a new parameter for the evaluation of the prognosis of AL patients.
出处
《江西医药》
CAS
2010年第4期305-307,共3页
Jiangxi Medical Journal
