摘要
首先采用正交试验法优化单宁酶高产黑曲霉的发酵条件,考察了单宁酸含量、KH2PO4含量、(NH4)2SO4含量、MgCl2.6H2O含量、温度、pH值、摇速7个因素对单宁酶产量的影响;再通过两步简便而有效的方法分离纯化出电泳纯的单宁酶,其纯度和分子量分别采用高效液相色谱(HPLC)和SDS-PAGE法测定。通过正交试验结果的方差分析,得出单宁酸含量对产酶量影响比较显著,并确定黑曲酶发酵产酶的最佳条件为:2%单宁酸,0.1%KH2PO4,0.7%(NH4)2SO4,0.1%MgCl2.6H2O,温度40℃,pH值5.6,摇速140r/min。SDS-PAGE法测定该酶的分子量为119ku。
The optimal fermentation conditions of tannase by Aspergillus niger were investigated using orthognnal experiment. The effects of tannic acid content, KH2PO4 content, （NH4）2SO4 content, MgCI2-6H20 content, temperature, pH and rotation speed on the yield of tannase were studied. High-performance liquid chromatography and SDS-PAGE were applied to analyze the purity and molecular weight, respectively. The result of or- thogonal analysis of variance showed that the effect of tannic acid content on the yield was significant. The optimum conditions of tannase production by Aspergillus niger were as follows： tannic acid 2%, KH2PO4 0.1%, 0.7%（NH4）2SO4, MgCi2 · 6H20 0.1%, temperature 40℃, pH 5.6, rotation speed 140 rpm. The molecular weight of pure tannase was 119 KD analyzed by SDS-PAGE.
出处
《中国酿造》
CAS
北大核心
2010年第5期109-113,共5页
China Brewing
关键词
正交设计
单宁酶
纯化
凝胶电泳
orthogonal design
tannase
purification
SDS-PAGE
