摘要
目的探讨大黄素对内毒素激活后巨噬细胞高迁移率族蛋白B1(HMGB1)基因表达和胞外释放的影响。方法内毒素脂多糖激活后小鼠腹腔巨噬细胞培养物经不同浓度(5、20、50μmol/L)大黄素作用一定时间,观察培养上清液HMGB1、TNF-α含量的变化及细胞HMGB1mRNA表达的改变。结果大黄素(50μmol/L)明显抑制脂多糖激活后小鼠腹腔巨噬细胞HMGB1mRNA的表达和HMGB1、TNF-α的释放(均P(0.01)。结论大黄素具有抑制巨噬细胞HMGB1mRNA表达和HMGB1胞外释放的作用。
Objective To study the effect of emodin on mRNA expression and extracellular release of high mobility group box 1（HMGB1）in endotoxin-stimulated macrophages.Methods The changes of HMGB1 and TNF-α levels in the culture medium and expression of HMGB1 mRNA were investigated after Emodin（5、20、50 μmol/L）was incubated with lipopolysaccharide-stimulated cultured mouse peritoneal macrophages.Results Emodin（50 μmol/L）markedly suppressed the release of HMGB1 and TNF-α,and expression of HMGB1 mRNA from lipopolysaccharide-stimulated macrophages（P﹤0.01）.Conclusion Emodin confers inhibition onto extracellular release of HMGB1 and expression of HMGB1 mRNA from endotoxin-stimulated macrophages.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2010年第1期87-89,共3页
Suzhou University Journal of Medical Science
基金
江苏省自然科学基金资助项目(BK2006027)
江苏省中医药局科研项目(H05178)
