肾八味胶囊对肾病综合征模型小鼠血清IL-6的影响被引量：1

Effect of Shenbawei capsule on serum IL-6 of mice with nephrotic syndrome

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摘要目的:观察肾八味胶囊对肾病综合征(NS)模型小鼠24h尿蛋白和血清白介素6(IL-6)的影响,以探讨该方对NS的免疫治疗作用机制。方法:给小鼠一次性尾静脉注射嘌呤霉素氨基核苷(Puromycin aminonucleoside,PA)复制NS模型,40只小鼠随机分为模型组、肾八味低剂量组、肾八味高剂量组、至灵胶囊组、生理盐水组,采用BCA法检测各组小鼠24h尿蛋白,采用酶联免疫吸附法检测小鼠血清IL-6的含量。结果:模型组小鼠24h尿蛋白和血清IL-6均较生理盐水组显著升高,中药灌服后能显著降低NS小鼠24h尿蛋白和血清IL-6的含量。结论:降低血清IL-6的含量可能是肾八味胶囊治疗NS的作用机制之一。 Objective：To explore the immune mechanism of Shenbawei capsule to treat nephrotic syndrome（NS） by observing 24 h urinary protein and serum interleukin-6（IL-6） of mice. Methods：Each mouse was given a single intravenous injection with puromycin aminonucleoside（PA） to make NS model. Fouty mice were randomly divided into 5 groups：model group,low-dose Shenbawei group,high-dose Shenbawei group,normal saline group,Zhiling capsule group. The 24 h urinary protein were measured by bicinchoninic acid assay（BCA） and contents of serum IL-6 detected by enzyme-linked immunosorbent assay. Resuhs：The 24 h urinary protein and serum IL-6 in model group were higher than those in normal saline group,The 24 h urinary protein and the contents of serum IL-6 were obviously decreased after treatment. Conclusion：Decreasing the level of IL-6 may be one of the mechanisms of nephrotic syndrome treated with shenbawei capsule.

作者桑素珍沈惠风部帅

机构地区上海交通大学医学院附属仁济医院中医科山东中医药大学

出处《山东中医药大学学报》 2010年第3期263-265,共3页 Journal of Shandong University of Traditional Chinese Medicine

基金上海市卫生局科研课题(编号:2006035)

关键词肾八味胶囊嘌呤霉素氨基核苷肾病综合征白介素6 实验研究 Shenbawei capsule puromycin aminonucleoside nephritic syndrome interleukin-6 experimental study

分类号 R277.592 [医药卫生—中医学]

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