摘要
目的:寻找连翘叶中α-葡萄糖苷酶抑制活性的成分。方法:利用体外α-葡萄糖苷酶抑制模型活性追踪,采用各种色谱法分离,运用多种波谱技术鉴定结构,并对活性较强的单体化合物进行酶抑制动力学研究。结果:连翘叶的乙酸乙酯部分具有较高的活性,从中分离出5个活性化合物,对α-葡萄糖苷酶抑制活性高于阳性对照阿卡波糖。酶抑制动力学反应结果表明,化合物1,4和1与2混合物(2∶1)对α-葡萄糖苷酶的抑制类型为非竞争性抑制剂。结论:化合物1～3为首次发现对α-糖苷酶的抑制活性,3为首次从连翘属中分离得到。
Objective： The α-glucosidase inhibitory Compounds were searched from Forsythia suspense. Method： The active compounds were isolated by the method of bioassay-guided in vitro and column chromatographic techniques. The structures of compounds were identified by MS and NMR spectroscopy. The inhibitory kinetics of the isolated compounds were investigated. Result： The ethyl acetate extract showed the strongest inhibitory activity, and five active compounds were isolated from this extract. The IC50 value of compound 1-5 were all lower than that of acarbose as positive control. Compound 1, the mixture of 1,2 and 4 all showed noncompetitive type model on α-glucosidase. Conclusion： Compound 1-3 as the inhibitors of α-glucosidase were reported for the first time. Compound 3 was isolated from the genus for the first time.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第9期1156-1159,共4页
China Journal of Chinese Materia Medica
基金
河南省教育厅基础研究计划项目(2008A360002)
河南省教育厅骨干教师计划(2008)
