摘要
为筛选出L-苏氨酸的高产菌,以谷氨酸棒状杆菌(Corynebacterium glu anicum)为出发菌,进行亚硝基胍(NTG)诱变处理选育试验。结果表明:筛选出蛋氨酸+赖氨酸+异亮氨酸缺陷型菌株Y-1(Met-+Lys-+Iso-),经摇瓶发酵72 h后,L-苏氨酸积累可达4.14 g/L。
Corynebacterium glu anicum was treated by nitrosoguanidine to breed strains producing high yield of L-threonine. The results showed that Y-l, a strain with Met^-＋Lys^ ＋Iso^- , was bred and its L-threonine accumulation amount was up to 4.14 g/L after 72 h shake-flask fermentation culture
出处
《贵州农业科学》
CAS
北大核心
2010年第4期107-109,共3页
Guizhou Agricultural Sciences
基金
江苏畜牧兽医职业技术学院院长基金项目"L-苏氨酸生产菌的研究"
