摘要
以黄色短杆菌ＡＴＣＣ１４０６７为出发菌株，经硫酸二乙酯，紫外线玫 硝基胍逐级诱变处理，α－氨基－β羟基戊酸，Ｓ－２－氨基乙基－Ｌ－半胱氨酸，磺胺胍，乙硫氨酸，α－氨基丁酸，异亮氨酸氧肟酸等氨基酸结构类似物及琥珀酸为碳源板定向筛选，获得一株Ｌ－异亮氨酸高产菌ＺＱ－４在含１３．５％葡萄糖培养基中，摇瓶发酵７２ｈ，Ｌ－异亮氨酸积累可达２．８－３．０％。
Brevibacterium flavum ATCC14067 as a starting strain was stepwise treated with diethylsufate(DES), ultraviolet (UV) and N-methyl-N'-nitrosonguanidine (NTG). A L-isoleucine over-producing mutant,ZQ-4,was obtained, which could resist to α-amino-β-hydroxyvalic acid (AHV),S-(β-aminoethyl)-L-cysteine (AEC), sulfaguanidine (SG), ethionine (Eth), α-amino-butyric acid (α-AB),isoleucine-hydroxamate (IleHx) and could grow quickly on succinate (as sole carbon source) medium. ZQ-4 could accumulate 2.8-3.0% of L-isoleucine in a medium containing 13.5% glucose shaking at 31±1℃ for 72hrs'.
出处
《氨基酸和生物资源》
CAS
1996年第3期1-5,共5页
Amino Acids & Biotic Resources
