摘要
目的研究下咽癌人咽鳞癌细胞(FADU)细胞系中CD133阳性细胞侵袭能力。方法用免疫磁珠分选技术,分选CD133阳性细胞及阴性细胞,Transwell小室方法研究阳性、阴性二个细胞亚群的体外侵袭能力。结果阳性、阴性细胞在Transwell小室侵袭实验中穿过人工基底膜的数量分别为54.0±2.3,17.0±1.1,其差异有统计学意义。结论下咽癌FADU细胞系中,CD133阳性细胞具有更强的侵袭能力。
Objective To investigate the invasion of the CD133-positive cells in the FADU cell line.Methods In our study,immunomagnetic beads were applied to purify CD133-positive cells in the FADU cell line.Invasions of CD133-positive and CD133-negative cells were measured by the Transwell experiment.Results The number of cells infiltrating the Transwell membrane in the CD133-positive group（54±2.3）was significantly higher than that in the CD133-negative group（17±1.1）.Conclusion CD133-positive cells in the FADU cell line show higher invasive ability.
出处
《山东大学耳鼻喉眼学报》
CAS
2010年第2期5-7,共3页
Journal of Otolaryngology and Ophthalmology of Shandong University
关键词
下咽癌
肿瘤干细胞
侵袭
CD133
Hypopharyngeal Cancer
Neoplastic Stem cells
Invasion
CD133
