摘要
在培养基中添加无菌TTC(三苯基四氮唑),最终质量浓度为2.5 mg/L,使菌落变红,方便观察计数。发现使用营养琼脂倾注法检验,至10-4稀释度才能消除样品中营养成分的干扰;而采用滤膜法,能有效消除饮料中营养成分的干扰,可以准确、灵敏地检验活性乳酸菌饮料中污染菌数,检测下限达到10 mL-1。整个货架期,蜡样芽孢杆菌、大肠埃希氏菌、沙门氏菌等污染菌在活性乳酸菌饮料中可以存活,建立准确简便的活性乳酸菌饮料菌落总数检测方法非常必要。
In the present study 2.5 mg/L sterile TTC was put into the medium to make the bacteria red for the convenience counting and found that the disturbance of the nutrient compounds could be vanished till 10^-4 dilution.However the filter membrane method could eliminate the dis turbance of the nutrition in the beverage to detect the contaminated bacteria accurately and effectively which detection lower limit can reach to 10 mL^-1.Due to the living of the contaminated bacteria including Bacillus cereus,Escherichia coli Salmonella etc.in the beverage during the shelf date,it is very impressive to set up an accurate and fast detection method.
出处
《中国乳品工业》
CAS
北大核心
2010年第4期54-56,59,共4页
China Dairy Industry
关键词
乳酸菌饮料
污染菌总数
检测培养基
检测方法
Lactobacillus beverage
total number of contaminated bacteria
detection culture medium
detection method
