摘要
[目的]研究三氯化铝侧脑室注射对apoE基因敲除小鼠空间学习和记忆能力的影响及相关蛋白代谢型谷氨酸受体-1(mGluR1)的表达。[方法]取经跳台试验、避暗试验和Morris水迷宫试验筛选的正常apoE基因敲除小鼠,用随机数字表法按体重随机分为2组:对照组和0.5%三氯化铝染铝组,每组8只。染铝组经侧脑室注射染毒,3μL/d,连续5d;对照组以相同容量生理盐水注射,染毒期结束后从第15天开始用跳台试验、避暗试验和Morris水迷宫试验判断2组的认知能力;染毒期结束20d后断头取脑组织,用Western-blot方法对mGluR1蛋白进行定量检测。[结果]①染铝组跳台和避暗潜伏期较对照组明显缩短(P<0.05);第2天错误次数明显增多(P<0.05);Morris水迷宫试验结果显示,染铝组找到平台的潜伏期较对照组明显增加(P<0.05),而空间探索试验中在目标象限停滞时间明显减少(P<0.05)。②染铝组mGluR1表达量较对照组降低,且差别有统计学意义(P<0.01)。[结论]铝可致apoE基因敲除小鼠学习和记忆能力障碍,且可使mGluR1蛋白的表达降低。
[Objective] To investigate the effects of aluminum chloride（AlCl3）,which was administrated by lateral cerebral ventricle injection ,on spacial learning and memory in apoE knock out mice,as well as their expression of metabotropic glutamate receptor-subtype 1（mGluR1）.[Methods] After being successfully trained by step through test,step down test and morris water maze（MWM）,16 mice were evenly and randomly divided into 2 groups ：control group and exposed group. Mice in the latter group were treated with 0.5% AlCl3 by lateral ventricle injection for 5 d,3 μL/d,while the controls were treated with normal saline in the same volume as the exposed group. Cognitive ability was evaluated by step through test,step down test and MWM on the 15th day after Al treatment for 5 d. Cerebral cortex was taken from the mice on 20th day after 5 d Al treatment. mGluR1 in cytoplasm was detected by western-blot,images were collected respectively by gelatum analyzing system and scanner.Data were analyzed by the gelatum analyzing system.[Results] ① Latent period of step down test and step through test in the exposed group were significantly decreased（P 0.05）,the error count was significantly increased in the second day（P 0.05）compared with the control group. In MWM the time of place navigation was significantly increaseed（P 0.05）,and the time of spatial probe was significantly shortened（P 0.05）compared with the control group. ②The expression of mGluR1 was significantly decreased in the 0.5% AlCl3 exposed group compared with the control group（P 0.05）.[Conclusion] The learning and memory ability and the expression of mGluR1 in apoE knock out mice are decreased obviously by AlCl3 lateral ventricle injection.
出处
《环境与职业医学》
CAS
北大核心
2010年第4期209-211,共3页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金资助项目(编号:30671777)
