摘要
[目的]探讨铝暴露对小鼠认知功能及脑组织β-淀粉样前体蛋白(β-APP)基因表达的影响。[方法]动物模型高、中、低剂量组每日用AlCl3含量分别是120、12、1.2mg/kg的饲料进行喂养,对照组每日正常饲养。染毒3个月后用跳台试验、避暗试验和水迷宫试验检测小鼠的认知功能。小鼠脑组织中β-APP mRNA的表达用逆转录-聚合酶链反应(RT-PCR)法检测。[结果]与对照组相比,高、中剂量组跳台试验潜伏期明显缩短(P<0.05),错误次数增多(P<0.05);避暗试验高剂量组潜伏期较对照组明显缩短(P<0.05),错误次数明显增多(P<0.05);水迷宫试验低、中、高剂量组随着染铝浓度的增加潜伏期明显延长(P<0.05),高、中剂量组穿越平台的次数与对照组相比明显减少(P<0.05)。高、中剂量组β-APP mRNA的表达量明显增高(P<0.05)。铝暴露剂量与跳台试验潜伏期(rs=-0.725,P<0.05)、避暗试验潜伏期(rs=-0.765,P<0.01)、Morris水迷宫试验潜伏期(rs=0.097,P<0.05)、跳台试验错误次数(rs=0.565,P<0.01)、避暗试验错误次数(rs=0.509,P<0.05)之间均存在剂量-效应关系。铝暴露与β-APP mRNA的表达量呈明显的正相关(rs=0.743,P<0.05)。[结论]铝暴露可导致小鼠认知功能障碍及β-APP的过度表达,这可能是铝致小鼠认知功能障碍的重要机制之一。
[Objective] To explore the effect of chronic aluminum exposure on cognitive ability and expression of β-APP gene in mice.[Methods] The high,middle and low dose groups were established by the gavages which the dosage of AlCl3 in the fodder were 120,12 and 1.2 mg/kg respectively.The control group was foddered regularly. Three months later,the spatial learning and memory ability were evaluated by step through test（STT）,step down test（SDT）,and morris water maze（MWM）,while gene expression of β-APP by RT-PCR（reverse transcription-polymerase chain reaction）.[Results] Compared with the control group,the latent period of SDT of high and middle dose groups was significantly decreased（P 0.05）,the error number of SDT of high and middle dose groups was significantly increased（P 0.05）.The latent period of STT of high dose group was significantly decreased（P 0.05）and the error number significantly increased（P 0.05）. In MWM the latent periods of different dose groups were significantly and constantly extended corresponding with doses of AlCl3（P 0.05）,numbers of traversing flat in high and middle dose groups were significantly decreased（P 0.05）. The expressions of β-APP gene in high and middle dose groups were significantly enhanced compared with the control group. There were dose-response relationships between aluminum exposure and the latent period of SDT（r = -0.725,P 0.05）,of STT（r = -0.765,P 0.01）,and of MWM（r = 0.097,P 0.05）. There were dose-response relationships between aluminum exposure and the error number of SDT（r = 0.565,P 0.01）,and of STT（r = 0.509,P 0.05）. Statistical analysis showed that positive correlations existed between aluminum exposure and expression of β-APP mRNA（rs = 0.743,P 0.05）.[Conclusion] The exposure to aluminum could result in the dysfunction of the ability of spatial learning and memory of mice and the over-expression of β-APP gene. It might be one of the most important pathogenic mechanisms.
出处
《环境与职业医学》
CAS
北大核心
2010年第4期205-208,共4页
Journal of Environmental and Occupational Medicine
