摘要
[目的]研究云锦杜鹃组织培养再生植株的最佳培养基配方条件,为其快速繁殖及体细胞杂交再生植株提供基础技术参考。[方法]以云锦杜鹃幼叶为材料,接种在MS、WMP、6,7-V3种基本培养基以及不同的激素组合、培养基不同磷素用量等各种处理中,重复4次进行离体培养,筛选其脱分化和再分化效果。[结果]发现WMP有利于诱导叶出愈伤,不定芽分化最佳的基本培养基为6,7-V;继代和增殖培养基为6,7-V+0.5mg/LZT+0.1mg/LNAA+0.1mg/LIAA,平均增殖系数可达12.25;试管苗最适生根培养基为1/26,7-V+0.1mg/LNAA+0.1mg/LIAA,30d的平均生根率达到91.20%。研究还发现,在培养基中提高磷素用量有利于再生芽的伸长。[结论]6,7-V培养基在云锦杜鹃幼叶不定芽发生中起显著作用,该研究建立了最适的云锦杜鹃离体再生体系。
[Objective] The basic technical reference for the rapid propagation and the plantlet regeneration of somatic hybridization of health family-Rhododendron was provided through the study on the best medium formula for its plant regeneration be means of the system of tissue culture.[Method] The Rhododendron young leaf as explants was inoculated in vitro on the 3 basic types of media： MS,WMP and 6,7-V with the different combinations of hormones,the different amounts of phosphorus and other processing and there were four repetitions in each treatment so that the de-differentiation and re-differentiation effect from these treatments were analyzed.[Results] It was found that the medium——WMP was conducive to the callus induction from leaf; the best basic medium for the differentiation of adventitious bud was 6,7-V; the medium for its subculture and multiplication was 6,7-V ＋ ZT 0.5 mg/L ＋ NAA 0.1 mg/L ＋ IAA 0.1 mg/L,with the average multiplication coefficient of up to 12.25; the best rooting medium was 1/2 6,7-V ＋ NAA 0.1mg/L ＋ IAA 0.1mg/L,with the average rooting rate reaching to 91.20% for 30 days’ culturing.It was also found that amount-increasing of phosphorus in the medium was favor to the elongation of regeneration bud.[Conclusion] A prominent role in induction of the adventitious buds from Rhododendron young leaf culturing was played by the medium——6,7-V and the optimal regeneration system of Rhododendron culturing in vitro was established.
出处
《安徽农业科学》
CAS
北大核心
2010年第11期6008-6010,共3页
Journal of Anhui Agricultural Sciences
基金
江西教育厅重点计划项目[赣教技(2005)84号]
