摘要
目的:125I标记Toll样受体4抗体(TLR4)研究其与人单核细胞系THP1细胞体外结合的特性。方法:用125I标记TLR4,以快速硅胶簿层层析纸(ITLC-SG)分析方法鉴定125I标记抗体的放射化学纯度,再用γ计数器测定125I标记抗体与THP1细胞的TLR4结合量,其中特异结合(SB)=总结合管(TB)-非特异管(NSB)。结果:125I标记TLR4-Ab的标记率为80.27%(n=7)。用快速硅胶簿层层析纸(ITLC-SG)分析方法鉴定其放射化学纯度都能>95%,测定125I标记抗体的比活度为3.145TBq.mg-1。结论:125I标记的TLR4-Ab具有良好的标记率和放射化学纯度,且125I标记TLR4-Ab与THP1细胞在体外具有很高结合亲和力。
Objective：Use of iodine-125 to label anti-humanTLR4 antibody and study its characteristic in human monocytic cell line THP1 in vitro. Methods：With iodine-125 to label anti-humanTLR4 antibody and the radiochemical purity was obtained by ITLC-SG. Then using the γcaculator to evaluate the binding value of iodine-125 labeled TLR4 antibody and the THP1 cells,SB（specific binding）=TB（total binding）-NSB（non-specific binding）. Results：The labeling efficiency of 125I-TLR4-Ab was 80.27%（n=7）. Radiospecific activity of 125I-TLR4-Ab had 3.145TBq·mg-1 and radiochemical purity（RCP） had a higher 95%. Conclusions：125I-TLR4-Ab possesses good labeling efficiency and perfect radiochemical purity.The affinity of 125I-TLR4-Ab with human monocytic cell line THP1 was high enough for detection in vitro.
出处
《中国临床医学》
2010年第2期155-158,共4页
Chinese Journal of Clinical Medicine
