摘要
为了阐明酒精中毒对神经系统损害的机理，用新生CD大鼠酒精中毒模型及原代培养的神经小胶质细胞作为研究对象，用RT－PCR的方法测定酒精中霉对脑组织及体外培养细胞中IL－1α基因水平的改变作用。结果显示：酒精持续染毒11d后，大脑皮层、小脑、海马、纹状体及丘脑组织中的IL－1α基因表达都有不同程度的增加，其中海马增加程度最为明显，是对照组的2．5倍；酒精对基底神经节组织IL－1α基因表达无影响。酒精也使体外培养的小胶质细胞在染毒24h后IL－1α基因表达量开始升高，72h达到高的。体内及体外实验均表明：酒精中毒时IL－1α的基因表达水平增加，继而调控神经和免疫功能，并可能在酒们导致的大脑神经损伤中起重要的作用。
In order to elucidate the mechanism of ethanol impairment on nerve system, we have used postnatal rat model and primarily cultured microglia to study the effect of ethanol on interleukin-la (IL-la) gene expression using reverse transcription and polymerase chain reaction (RT-PCR) assay. The results showed that postnatal CD rats received ethanol intubation for 11 days resulted in increased IL-la gene expression in cerebral cortex, cerebellum, hippocampus, striatum and thalamencephalon. Hippocampus presented the greatest level of ethanol--induced increased IL-la gene expression f 2. 5-fold greater than non-treated control group, whereas hasal Ganglia was not affected. The ethanol-induced increase of IL--la gene expression was also found in primary microglia cultures after cell were exposed to ethanol for 24 h. The effect reached a plateau after 72 h of ethanol treatment. Results from our in vivo and in vitro studies suggest that ethanol-induced increased IL-la gene expression may associated with the regulation of neuro-and immuno-systems,and may play important role ethanol-induced brain damage.
