摘要
应用原位杂交、放射自显影结合计算机图像处理技术检测电针（EA）后10h及24回时大鼠中枢多巴胶（DA）D1和D5受体信使RNA（mRNA）的变化．结果显示：EA后10h脊瞩D1和D5受体mRNA有明显增高，24h时进一步增高；而脑内D1和D5受体mkNA需至24回时才有显著增高。结果提示，在转录水平，EA可加强大鼠中枢D1和D5受体基因的表达，使这些受体合成增加。
In situ hybridization and autoradiographic techniques were used to detect the changes of central D1 and D5 receptor messenger RNA (mRNA) at 10 b and 24 h after electro-atupuncture (EA). The resultSshowed that the D1 and D5 receptor mRNA in spinal cord increased at 10 h and further increased at 24 k afterEA, however, the obvious Increase or brain D1 and D5 reteptor mRNA was observed only at 24 b after EA. TheresultS indicate that the gene expression of central D1 and D5 receptor can be enhanced by EA,suggesting theincrease in synthesis of these receptors.
基金
国家自然科学基金!No.39230360
上海市科委基金!No.95×D14004
关键词
针刺
镇痛
多巴胺受体
原位杂交
放射自显影
acupuncture analgesia
dopamine receptor
in situ hybridization
autoradiography
