摘要
通过单因素试验研究了基于黄芩苷PKa值的酸醇超声提取法的工艺条件,并用正交试验进行了优化,最优提取工艺条件为:黄芩粗粉(约40目),pH 4.0的60%乙醇液18倍量,60℃、32 kHz超声提取2 h,合并滤液,浓缩至1/3,调pH值至2.0,80℃保温静置4 h,离心,洗涤,60℃干燥5 h,黄芩苷提取率10%以上,含量85%以上.
Using acid-ethanol solution as extractant, the extraction of from Scutellaria ba- icalensis Georgi. by ultrasonic wave based on PKa was studied by single-factor experiments and optimized by orthogonal test, the optimal conditions were as follows ： the powder （filtered by 40 meshes） of Scutellaria baicalensis Georgi. was extracted by as much as 18 times volume of 60% ethanol solution under pH 4.0 with ultrasonic wave of 32 kHz for 2 h. After filtering, the filtrate was collected and condensed to 1/3 volume. Fitted the pH to 2. 0, standing for 4 h under 80℃, then centrifugated, washed, and dried for 5 h at 60℃. The extraction rate was above 10% and the baicalin content was up to 85%.
出处
《陕西科技大学学报（自然科学版）》
2010年第2期73-76,共4页
Journal of Shaanxi University of Science & Technology
