摘要
对冬枣贮藏期引发黑斑病、浆胞病和轮纹病的9株真菌菌株进行了分子水平鉴定。采用结合病原物形态特征和rDNA ITS区序列特点的方法对获得的菌株进行分析。研究发现,冬黑2(DH2)、冬黑4(DH4)、冬浆3(DJ3)、冬浆4(DJ4)、轮纹3(LW3)和轮纹5(LW5)在PDA培养基与玻片上均产生孢子;轮纹4(LW4)有明显产孢结构及椭圆形到卵圆形单细胞的分生孢子;冬黑4(DH4)、冬浆3(DJ3)、冬浆4(DJ4)和轮纹5(LW5)产生砖格状分生孢子;冬黑1(DH1)和冬浆5(DJ5)经处理和诱导后仍不产孢。以ITS1和ITS4为引物,对9株真菌的基因组进行扩增,得到约550bp左右的ITS区rDNA片段。将测序结果登陆GenBank进行BLAST分析,依据Neighbor-joining方法构建系统发育树。形态与分子鉴定结果表明,供试的9个菌株中,冬黑4(DH4)、冬浆3(DJ3)、冬浆4(DJ4)和轮纹5(LW5)为链格孢属(Alternaria spp.);冬黑1(DH1)和轮纹4(LW4)为拟茎点霉属(Phomopsis spp.);冬黑2(DH2)为茎点霉属1种(Phoma sp.);轮纹3(LW3)为嘴突脐蠕孢种(Exserohilum rostratum);冬浆5(DJ5)为匍柄霉属1种(Stemphylium sp.)。
Nine pathogenic fungal strains on Dongzao jujube in China were identified and analyzed according to the morphological characteristics and ITS sequences. Morphological studies showed that strains DH2,DH4,DJ3,DJ4,LW3 and LW5 generated spores on potato dextrose agar plates and slides. Strain LW4 generated distinct conidiomata and single-celled oval conidia. Strains DH4, DJ3, DJ4 and LW5 produced muriform conidia. The rDNA ITS regions of these pathogens were amplified by PCR and then sequenced. After analyses of their ITS sequences, a phylogenetic tree was obtained from the program NJ （Neighbor-joining）, together with the sequence of related strains which were downloaded from GenBank. The results of morphological observation and molecular detection of these nine strains showed that DH4, DJ3, DJ4 and LW5 belong to Alternaria spp. ; DH1 and LW4 belong to Phomopsis spp. ; DH2 belongs to Phoma sp. ; LW3 belongs to Exserohilum rostratum and DJ5 belongs to Sternphylium sp.
出处
《果树学报》
CAS
CSCD
北大核心
2010年第3期422-426,共5页
Journal of Fruit Science
基金
山西省科技攻关项目(20080311046-1)
山西省农业科学院博士基金(YBSJJ0703)
关键词
冬枣
病害
形态特征
ITS序列分析
鉴定
Dongzao jujube
Diseases
Morphological characteristics
ITS sequence analysis
Identification
