摘要
目的:探讨色胺酮(Tryptanthrin,Try)对人乳腺癌MCF-7细胞增殖和凋亡的影响。方法:利用MTT方法检测Try(1.56-100μmol/L)对细胞增殖的影响;透射电镜观察细胞的形态学改变;流式细胞术检测细胞周期、凋亡情况及线粒体跨膜电位等指标。结果:MTT结果显示,Try在12.5-100μmol/L浓度范围内能明显抑制MCF-7细胞的增殖,并具有时间和浓度依赖性;透射电镜下可见Try作用48h后,MCF-7细胞有典型的凋亡样改变。Annexin V-FITC与PI双染,流式细胞仪检测结果显示:50、100μmol/LTry作用后,细胞的凋亡情况明显,与对照组相比差异显著;且影响了MCF-7的细胞周期分布,将细胞阻滞于G1期,抑制其DNA的合成;并导致细胞线粒体跨膜电位下降。结论:色胺酮能明显抑制MCF-7细胞增殖并具有诱导细胞发生凋亡的作用。
Objective： To study the effect of tryptanthrin（Try） on proliferation and apoptosis of breast cancer MCF-7 cells. Methods： The proliferative effect of Try （1.56-100 μmol/L） on MCF-7 cells was assessed by MTT assay. The morphologic change was observed by transmission electron microscope. The flow cytometer was used to detect the changes of cell apoptosis, cell cycle and mitochondria membrane potential. Results： MTT results showed that in the range of 12.5-100μ mol/L, Try obviously inhibited the proliferation of MCF-7 cells in a dose and time-dependent manner. Typical apoptosis changes were observed in MCF-7 cells treated with Try for 48 h by transmission electron microscope. To stain with Annexin V-FITC and PI, Flow cytometer results showed that the apoptosis state was obvious in the cells treated with 50, 100 μmol/L Try for 48 h, respectively. Try can change the cell cycle distribution of MCF-7 cells. The G 1 phase was blocked and the DNA synthesis was inhibited. Meanwhile, the mitochondrial membrane potential was decreased. Conclusions： Try has an effect on inhibiting the cell proliferation and inducing the apoptosis of MCF-7 cells.
出处
《现代生物医学进展》
CAS
2010年第6期1065-1068,共4页
Progress in Modern Biomedicine
