摘要
目的:利用基因表达谱与基因功能知识识别虚寒证相关的差异基因与功能模块,从功能模块水平研究虚寒证发生的分子机制。方法:用Array Tools筛选差异表达基因,并检验分类效果,再利用Gene Ontology(基因本体,GO)工具,将特征基因进行功能注释,选择显著聚集差异表达基因的功能模块;识别其中能够有效分类疾病样本的特征功能模块,并分析它们与虚寒证的关联。结果:用25个差异基因分类样本准确率在93%,用11个功能模块分类样本准确率在87%-100%之间。结论:这些特征功能模块与虚寒证的发生有一定的关联性。基于特征功能模块法研究基因表达谱更有利于对疾病的阐释。
Objective：To identify the deficient cold syndrome relevant genes and functional modules,based on gene expression profiles and gene functional knowledge to study the molecular mechanism of deficient cold syndrome.Methods：Screening the differential expression genes with Array Tools,and testing the classificational results to the samples.The Gene Ontology Tool was used for annotating characteristics functional gene,selecting functional modules enriched with differentially expressed genes;and then identifing the feature modules and analyzing their association with the deficient cold syndrome.Results：The accuracy rate of classification samples was 93% with 25 different gene,the accuracy rate was 87%-100% with 11 functional modules.Conclusion：These features function modules had some relevance to the occurrence of deficient cold syndrome.It was more favorable interpretation of the disease by studying functional modules based on gene expression profiles.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2010年第5期683-685,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金海外青年学者合作项目(No.30528029)
河南省医学创新人才工程项目(No.2005075)
河南中医学院博士基金项目(No.2007005)~~
关键词
基因表达谱
基因本体
功能模块
特征基因
Gene expression profi le
Gene Ontology
Functional module
Feature gene
