摘要
目的:建立妇康健合剂中柴胡苷a的含量测定方法。方法:用3%的吡啶甲醇提取,用大孔树脂(DA-201)纯化样品,并用高效液相色谱法测定妇康健合剂中柴胡皂苷a的含量。色谱柱:Shimadzu ODS(150 mm×4.6mm,5μm),流动相:乙腈-水(30:70v/v),流速:1.0 ml·min^(-1),检测波长:208 nm,柱温:35℃。结果:柴胡皂苷a在1.08～8.64μg范围内有较好的线性关系(r=0.999 8),平均加样回收率为98.3%,RSD为1.4%。结论:本方法可作为妇康健合剂中柴胡皂苷a的含量测定方法。
Objective： To establish an HPLC method for quantitative determination of fukangjian oral liquid . Method： 3% pyridine methanol was used to extract and DA-201 maeropous resin was usecl to purity samples, the content of saikosaponin a in fukangjian oral liquid was determined by HPLC. The determination was performed on Shimadzu ODS （ 150 mm× 4.6mm,5 μm） column, the mobile phase consisted of aeetonitrile - water （30： 70, v/v）with flow rate was 1.0 ml @ min^-1, detection wavelength at 208 nm and the column temperature was 35 ℃ o Result： Saikosaponin a showed a good linear relationship in the range of 1.08 - 8.64 μg, r = 0.999 8, and the average recovery was 98.3% with RSD of 1.4%. Conclusion： This method can be applied to reference for quality control of fukangjian oral liquid.
出处
《中国药师》
CAS
2010年第5期699-700,共2页
China Pharmacist
关键词
妇康健合剂
柴胡
含量测定
大孔树脂
Fukangjian oral liquid
Radix bupleur
Quantitative determination
Macropous resin
