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嗜盐菌群在甲苯降解过程中群落结构的变化解析 被引量:5

Analysis of bacterial community structure in toluene degradation by a halophilic consortium
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摘要 从胜利油田的高盐油污土壤中富集到1个高效降解BTEX(苯、甲苯、乙苯、二甲苯)的嗜盐菌群,分析了菌群在甲苯降解过程中的群落结构变化.结果表明,该菌群在5%盐度下可完全降解200mg/L甲苯.PCR-DGGE图谱显示,随着甲苯的加入,该菌群的优势种属由Bacillus sp.和Bacillus aquimaris转变为Thalassospira xiamenensis、Pseudomonas stutzeri、Virgibacillus sp.和Bacillus sp..这4种微生物在整个降解过程中稳定存在,且在降解完成后24h内没有衰亡的迹象.另外,该菌群可以降解菲,显示了降解多种芳香族化合物的能力. A halophilic bacterial consortium that could degrade BTEX(benzene,tolunene,ethybenzene,xylene) at the salinity of 5% was enriched from oil-contaminated saline soil.Bacterial community structure dynamics in toluene degradation by the consortium was analyzed.The consortium could degrade 200 mg/L toluene at the salinity of 5%.PCR-DGGE profiles revealed that with toluene addition,the predominant species shifted from Bacillus sp.and Bacillus aquimaris to Thalassospira xiamenensis,Pseudomonas stutzeri,Virgibacillus sp.as well as Bacillus sp.,four of which remained stable during the degradation process and the following 24h when toluene was depleted.In addition,the consortium could degrade phenanthrene,illustrating its versatility in utilizing aromatic hydrocarbons.
出处 《中国环境科学》 EI CAS CSCD 北大核心 2010年第2期216-221,共6页 China Environmental Science
基金 国家自然科学基金资助项目(30970098,2073001) 国家重点实验室专项基金课题(09Y03ESPCT)
关键词 BTEX 嗜盐菌群 PCR-DGGE 微生物群落结构 BTEX bacterial halophilic consortium PCR-DGGE microbial community structure
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二级参考文献122

共引文献135

同被引文献42

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