摘要
土壤样品采自沈阳东部石油污水灌渠的上、中、下游。进行了污染物 (Cd、矿物油 )含量分析和生态毒性试验。重金属采用原子吸收分光光度仪测定 ,矿物油采用紫外分光光度计测定。生态毒性试验分别参照国际标准组织 (ISO)和OECD指南 ,进行了植物毒性试验和蚯蚓毒性试验。植物试验以小麦种子发芽根伸长抑制率为试验终点 ,试验周期 5 0h ,蚯蚓毒性试验以蚯蚓死亡率和体重增长抑制率为试验终点 ,试验周期 2 8d。结果表明 ,所有土壤均有矿物油积累 ,含量为 6 6 2 2～ 1391 14mg·kg-1,重金属Cd略高于土壤背景值 ,含量为 0 0 6～ 0 37mg·kg-1。然而 ,土壤对高等植物表现有不同程度的生长刺激效应 ,对蚯蚓有急性致死及慢性亚致死效应。种子发芽根伸长抑制率为 - 2 8 6 1%～ - 5 4 6 5 % ,蚯蚓 2 8d死亡率最大值为 10 %。蚯蚓 14d和 2 8d体重增长抑制率分别为 - 5 13%～ - 2 8 0 5 %和 - 31 78%～ 8 0 8%。研究表明 ,长期石油污水灌溉土壤中矿物油的低积累和较明显的生态毒性。
Soil samples were collected from several sites in the up,middle and dowstream along the petrol-wastewater irrigation channel,eastern Shenyang.Concentrations of heavy metals (Cd) and mineral oil were analyzed.The eco-toxicity of soil was evaluated.Heavy metals (Cd) were determined by AAS (atom adsorption spectrometer),mineral oil was measured by UV spectrometer.The eco-toxicity of soil was based on the method of international standardization of organization (ISO) and OECD guideline with some modification.With inhibition rates of root elongation of wheat as endpoint' seed germination test was performed and the duration period was 50h.With mortality rate and inhibition rate of weight growth as endpoint' earthworms test was conducted and the experimental period was 28d.Results showed that all soil tested have low accumulation of mineral oil measured with concentration of 66.22～1391.14 mg·kg -1.Cd was slightly higher than the background value with content of 0.06～0.37mg·kg -1.Soil showed a strong stimulation effect on root elongation of plant,and a lethal and sub-lethal effect on earthworms was observed.The stimulation rate of root elongation based on germination test was -28.61% to -54.65%.The mortality rates of earthworms at maximum were stimulated and then 10%.The body weight growth were stimulated and then inhibited from -5.13% to -28.05% in 14d,and -31.78% to 8.08% in 28 d.Study indicated that long-term of irrigation with petroleum wastewater resulted in the low residue of mineral oil and observed eco-toxicity.
出处
《生态学杂志》
CAS
CSCD
北大核心
2004年第5期61-66,共6页
Chinese Journal of Ecology
基金
国家自然科学基金重点项目(20337170)
国家自然科学基金项目(20077029
20277041)
沈阳大学沈阳环境工程重点实验室基金
中国科学院沈阳应用生态研究所海外留学基金资助项目
关键词
土壤
污水灌溉
生态毒性
蚯蚓
高等植物
sediment,wastewater irrigation,eco-toxicity, earthworms,higher plants.
