摘要
建立固相萃取液相色谱法净化和测定枸杞中甜菜碱的分析方法。方法采用混合型阳离子交换小柱(Waters Oasis MCX)净化枸杞样品提取液,使用Atlantis HILIC色谱柱,以乙腈-水为流动相进行梯度洗脱,在195nm波长处检测。结果表明,线性范围为0.2～5.0mg/mL,方法检出限为0.0066mg/mL,平均回收率为88.3%～104%。本方法能简便、快速、准确地检测枸杞中甜菜碱。
A solid phase extraction and high-performance liquid chromatographic（SPE-HPLC） method was developed for the determination of betaine in the fruits of Lycium barbarum L..The cleanup of sample extracts was achieved on Waters Oasis MCX cartridge.A mixture of acetonitrile and water was used as the mobile phase for the chromatographic separation on Atlantis HILIC column in the gradient elution mode.The detection wavelength was set at 195 nm.The method exhibited a limit of detection of 0.0066 mg/mL and a linear range of 0.2-5.0 mg/mL.Average recoveries of three replicates for betaine in a real sample spiked at the levels of 0.05,0.1 mg/mL and 0.2 mg/mL ranged between 88.3% and 104%.A simple,rapid and accurate determination of betaine in the fruits of Lycium barbarum L.was successfully achieved using the method.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第8期199-201,共3页
Food Science
基金
宁夏回族自治区自然科学基金项目(NZ0748)
关键词
固相萃取
高效液相色谱法
枸杞
甜菜碱
solid phase extraction
high-performance liquid chromatography
Lycium barbarum L.
betaine
