摘要
建立辣根素在几种仓库贮藏品中的毛细管气相色谱残留分析方法。以二氯甲烷为提取溶剂,毛细管气相色谱-火焰离子化检测仪(FID)进行测定。当各样品中辣根素的添加量为1、5、25mg/kg时,平均回收率为70.6%～92.3%、相对标准偏差为3.6%～9.3%,辣根素在各样品中的最低检出量低于0.1mg/kg。辣根素在贮藏品中的消解动态符合一级动力学方程,其半衰期为28～75d,其降解过程主要是酶解、水解、光解;当辣根素施用质量浓度为25mg/L或者100mg/L时,贮藏112d后的水稻种子、小麦种子、大米、烟草、红枣、木耳的辣根素残留量分别为11.8、150.7,2.8、77.5,3.3、8.2,5.6、189.2,3.6、26.9,1.2、10.2mg/kg。质量分数20%辣根素油剂用于熏蒸仓库害虫防治的建议使用质量浓度为25mg/L,熏蒸后的安全间隔期为14d。
A method using capillary gas chromatography was presented for the determination of allyl isothiocyanate（AITC） residue in agro-products fumigated with it.Samples were extracted with dichloromethane.A capillary gas chromatography system equipped with flame ionization detector（FID） was used.Mean recoveries of AITC in a sample spiked at the levels of 1,5 and 25 mg/kg ranged between 70.6% and 92.3%,with relative standard deviations（RSDs） within the range of 3.6%-9.3%.The presented method exhibited a limit of determination of 0.1 mg/kg.The degradation of AITC in fumigated agro-products including rice seeds,wheat seeds,commercial rice,Chinese date and Jew＇s-ear,as a result of enzymolysis,hydrolysis or photolysis,complied with first-order kinetic equations,with half-life periods ranging from 28 to 75 d.After 112 d storage,the AITC residues in fumigated rice seeds,wheat seeds,rice,Chinese date and Jew＇s-ear were 11.8,150.7,2.8,77.5,3.3 mg/kg and 8.2 mg/kg at the treatment level of 25 mg/L and 5.6,189.2,3.6,26.9,1.2 mg/kg and 10.2 mg/kg at the treatment level of 100 mg/L.Therefore,the dosage of 20% dichloromethane solution of AITC should be set at the level of 25 mg/L.After 2 d sealed AITC fumigation,at least 14 d air exposure of agro-products is required for the degradation of most AITC residue.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第8期163-166,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD06B10)
关键词
烯丙基异硫氰酸酯
熏蒸
残留分析
降解动态
allyl isothiocyanate
fumigation
residual analysis
degradation kenetics
