摘要
PCR-DGGE技术是近年来新兴的一项开展微生物分子生态学研究的工具。本文介绍了PCR-DGGE技术的基本原理和技术背景,总结了应用PCR-DGGE研究发酵食品和饲料中真菌菌群时所设计或选择的PCR引物,图示了获得DGGE图谱中DNA条带序列信息的实验流程,并列举了DGGE技术的一些局限性和优化策略。综述结果有助于建立可行的PCR-DGGE技术方案用于发酵过程或产品中真菌群落结构和演替规律的检测分析。
PCR-DGGE technique is a recently emerging tool in microbial ecology.In this paper,the principle and background of PCR-DGGE are introduced.Meanwhile,the design and choice of PCR primers are summarized in investigation of fungal community in fermented food and feedstuff.Moreover,the experimental procedure to obtain DNA sequence information from DGGE profiles is demonstrated.Furthermore,limitations and optimization strategies of DGGE are also listed.This paper will be helpful for establishing a feasible PCR-DGGE protocol to detect fungal community structure and deduce succession pattern during fermentation process or in fermented products.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第7期317-322,共6页
Food Science
基金
湖南省重大科技专项(2008FJ1005)
关键词
变性梯度凝胶电泳
发酵食品
真菌菌群
PCR引物
denaturing gradient gel electrophoresis（DGGE）
fermented foodstuff
fungal community
PCR primers
