摘要
目的探讨大鼠重症急性胰腺炎模型制备技术的改进。方法60只健康SD大鼠随机分为改进组(A组,n=30)和对照组(B组,n=30)。A组采用一次性BD胰岛素注射器以5～10°角(针头与肠胰平面夹角)、B组采用一次性1ml注射器以10～15°角,分别经十二指肠乳头逆行胰胆管穿刺注射5%牛磺胆酸钠诱导大鼠重症急性胰腺炎模型。各组大鼠分别于术后24h处死,观察胰腺大体及光镜下病理改变,计算诱导成功率、24h存活率及肠坏死率,并进行统计学比较。结果两组大鼠胰腺均呈出血坏死性改变,光镜下均可见炎性细胞浸润。与对照组相比,改进组肠坏死率降低(P<0.05)。结论应用此方法对逆行胰胆管穿刺注射法制作大鼠急性重症胰腺炎模型加以改进,具有创伤小,操作简单,模型肠坏死率低等优点,是一种较理想的制模方法。
Objective To study related technique improvement on traditional animal model with severe acute pancreafitis in rats. Methods The animals were randomly divided into Group A and Group B. Severe acute pancreatitis was induced in Group A and Group B by retrograde infusion of 5% sodium taucrocholate into the biliopancreatic duct through the duodenal papilla with BD insulin syringes disposable and disposable lml syringes separately, with the angles between the needle and the intestinal pancreatic plane in group A and B were 5-10°and 10-15°separately. Rat in each group were killed at 24h after the operation to observe the general and the pancreas pathological changes under light microscope and to calculate the rates of successful induction, 24h survival, and intestinal necrosis, which were statisticaUy compared. Results Hemorrhagic and necrotic changes were showed in the pancreas of the rats, and inflammatory cells were seen infiltrated in the pancreas of the two groups in light microscope. Compared with group B, the rate of intestinal necrosis of group A were lower （ P〈 0.05）. Conclusions Using the method mentioned above to model animals of severe acute pancreatitis, with less trauma, simple operation, inducing the model of low rate of intestinal necrosis, is an ideal molding approach.
出处
《合肥医学院学报》
2009年第6期570-572,582,共4页
Journal of Zunyi Medical University
