摘要
本文采用ＨＰＬＣ技术研究了光强、温度、盐度三因子对盐藻积累β－胡萝卜素的动态协同胁迫作用．结果表明：它们存在着很强的协同胁迫作用，在温度２５℃，自然光照射时，２４０‰盐度积累β－胡萝卜素量大于１８０‰（０．７１６μｇ／ｍｌ＞０．３３７μｇ／ｍｌ），但在３２℃，光强１００００ｌｘ时，１８０‰盐度积累量大于２４０‰（（２．９４８μｇ／ｍｌ＞０．９８８μｇ／ｍｌ）；这一动态过程的时间曲线为“抛物”曲线；强胁迫条件有利于全反式异构体积累。
The paper use high performance liquid chramatography(HPLC)to research the dynamic rule of Dunaliella salina accumulating β-carotene under three stress factors(light intensity,temperature,salinity). These results suggest that three stress factors have strong interaction each other for Dunaliella salina to accumulate β-carotene,under the condition of 25℃ with indoor nature light the algae accumulate β-carotene(0.716μg/ml)in salinity 240‰ is more than in salinity 180‰(0.337μg/ml),under the conditions of 32℃ with light intensity 10000lx,the accumulating amount of β-carotene by algae in salinity 180‰(2.948μg/ml)is more than in salinity 240‰(0.988μg/ml);The time-curve which mirror the dynamic process about accumulation of β-carotene by Dunaliella salina is a sphape of parabola;The strength of stress conditions for accumulation of trans-isomer is stronger than cis-isomer.
基金
国家自然科学基金
浙江省自然科学基金
关键词
盐藻
Β-胡萝卜素
协同胁迫
异构体
HPLC
dunaliella salina teodoresco
co-force
ismoer
high performance liquid chramatogrphy(HPLC)
