摘要
以亚心形扁藻为实验对象,分别以NaNO3、CO(NH2)2和(NH4)2SO4作为氮源,KH2PO4和NaH2PO4作为磷源培养亚心形扁藻,并进行观察和计数,探讨不同氮源和磷源对亚心形扁藻生长的影响。结果显示,培养液中NaNO3、CO(NH2)2、(NH4)2SO4、KH2PO4和NaH2PO4都能促进亚心形扁藻的生长,以CO(NH2)2和NaH2PO4实验组促生长效果较为明显,亚心形扁藻的生长速率CO(NH2)2(0.0 512)>(NH4)2SO4(0.0 325)>NaNO3(0.0 306);在正交实验中,以CO(NH2)2和NaH2PO4为氮、磷源的营养盐组合能够显著促进亚心形扁藻生长,该实验组指数末期藻细胞浓度达到6.1×105cell/mL,是亚心形扁藻作为生物饵料的理想投喂浓度。
Through observing and counting the quantity of algae cells, the article discussed the effect on thegrowth rate of the algae from different nitrogen and phosphorus sources using Platymonus subcordiformis as the experimental object, sodium nitrate, urea or ammonium sulphate as nitrogen source, potassium dihydrogen phosphate or sodium dihydrogen phosphate as phosphorus source. The results showed that in the culture solu- tion of sodium nitrate, urea, ammonium sulfate, potassium dihydrogen phosphate and sodium dihydrogen phosphate, platymonas subcordiformis could grow normally, especially grow significantly in the urea and sodi- um dihydrogen phosphate solution. Urea (0. 0 512) is more effective than sodium nitrate (0. 0 325 ) and am- monium sulphate (0.0 306) on the growth rate of the algae. In the orthogonal experiment, using disodium hydrogen phosphate and urea as the nitrogen and phosphorus source could significantly promote the algae growth, and at the end of index growth period, the algae cell concentration could reach up to 6. 1 × 105cell/ mL, which was the most ideal bait concentration of flat algae used as live foods.
出处
《福建水产》
2014年第4期258-263,共6页
Journal of Fujian Fisheries
基金
国家海洋局--海洋公益性行业科研专项(编号:201205021-02)
关键词
亚心形扁藻
氮源
磷源
生长速率
Platymonus subcordiformis
nitrogen source
phosphorus source
growth rate