摘要
为进一步探讨紧张、压抑等心理因素诱发、加重银屑病的物质基础以及银屑病发病的神经免疫机制。采用ＲＩＡ测定了４９例银屑病患者及３４名正常人的血清对正常小鼠淋巴细胞转化的影响。４９例银屑病患者中男３１例，女１８例；年龄１４～７２岁，平均３８２岁；病程：１个月～３０年，平均病程７４年；皮损面积与占体表面积的百分比：５％１０例，６％～２０％１９例，＞２０％２０例；进行期２８例，静止期２１例。４９例患者在试验前一周均无药物治疗。３４名正常人，其中男２６例，女８例，年龄２２～６３岁，平均３４．５岁。此外还用神经免疫蛋白（ＮＩＰ）单克隆抗体中和银屑病患者血清，然后对正常小鼠淋巴细胞转化的抑制作用的试验。结果显示银屑病患者血清对正常小鼠淋巴细胞的转化率为４１．５５％，正常人血清对正常小鼠淋巴细胞的转化率为６８．０９％。表明银屑病患者血清中有抑制正常小鼠淋巴细胞转化的物质，进行期患者血清中的抑制作用大于静止期，皮损面积愈大者其血清对小鼠淋巴细胞转化率的抑制作用愈强（Ｐ＜０．００１，ｒ＝０．４９３９）。ＮＩＰ是在中枢神经系统控制下由外周淋巴组织产生的一种调节蛋白，释放入血，具有抑制淋巴细胞转化及其它免疫抑制作用。１９９１年?
In order to further investigate on the material basis of induction and aggravation of psoriasis by psychological factors like tension,repression etc,we have adopted RIA to determine the influence on the transformation of normal mice's lymphocyte in serum from 49 psoriatic patients ,and 34 normal volunteers Among 49 patients (31 males and 18 females,aged 14 to 72 years,with average age of 38.2,course of disease from 1 month to 30 years,averaging 7.4 years),28cases in progressive stage,21 in the stable stage,ratio of lesion area to body surface area:10 cases less than or equal to 5%,19 cases between 6% and 20%,20 cases greater than 20%. All the patients have not received medical treatment a week before study. At the same time,there were 34 healthy volunteers in normal group,26 male and 8 female,aged 22 to 63 years with average age of 34.5. Monoclonal antibody of Neuro-Immune Protein (NIP) was used to neutralize the serum from psoriatic patients to study its suppressive effect to lymphocyte transformation in mice. Results showed that the transformation ratio of the lymphocyte in serum from psoriatic patients was 41.55% but it was as high as 68.09% in serum from normal control group,It sugvip that there is certain substance which suppresses the transformation of normal mice's lymphocyte in serum from psoriatic patients. The suppressive effect of this substance at the progressive stage is stronger than it at the stable stage,moreover,the lesion area greater,the suppressive effect is stronger ( P <0.001, r =0.4939). NIP is a regulatory protein produced by peripheral lymphatic tissues under the control of central nervous system control,which is released into blood stream with the suppressive function of the transformation of lymphocyte and other immunosuppressive functions. In 1991,Farber et al presented the psychoneurotic immune mechanism pathogeny of psoriasis,considering that intensive life events have influence on nervous system,endocrinous system and immune system. The psoriatic patients have obvious immunologic functional abnomality. The relatively identical results come from immunologic study is low cellular immune function,low transformation ratio of peripheral blood lymphocyte,low ratio of natural petal foramation and delayed reaction type of skin hypersensitivity test. The results of this paper suggest that the substance which suppresses the transformation of lymphocyte exists in the serum of psoriatic patients. We conclude that this substance is similar to NIP by monoclonal NIP antibody neutralized experiment. But,the causality of the NIP and the pathogenes of psoriasis needs further study.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1998年第6期124-126,共3页
Journal of Jinan University(Natural Science & Medicine Edition)
