摘要
阐明人乳头瘤病毒１６型（ＨＰＶ－１６）、巨细胞病毒（ＣＭＶ）和单纯疱疹病毒２型（ＨＳＶ－２）感染与慢性宫颈炎的关系，考核重组人α１ｂ型干扰素治疗慢性宫颈炎的疗效和阻断ＨＰＶ－１６母婴传播的作用。方法：采用ＤＮＡ分子杂交技术、病毒分离技术，ＰＣＲ扩增技术检测标本。标本采自糜烂、正常宫颈，妊娠３９周的孕妇宫颈以及新生儿咽拭子。结果：通过对正常宫颈和糜烂宫颈ＨＰＶ－１６，ＨＳＶ－２和ＣＭＶ病毒检出率的比较，证明病毒感染与慢性宫颈炎高度相关；采用重组人α１ｂ型干扰素（ｒｈＩＦＮ－α１ｂ）治疗慢性宫颈炎一个疗程后，有效率达９３８％，显效率达６０％；ＨＰＶ－１６和ＨＳＶ－２的病毒检出率也相应明显下降。在干扰素治疗后，ＨＰＶ－１６的母婴传播率从８０％下降至２９４％。中药黄芪与干扰素有协同作用。结论：１）慢性宫颈炎与ＨＰＶ－１６，ＨＳＶ－２和ＣＭＶ病毒感染有关；２）ｒｈＩＦＮ－α１ｂ对治疗慢性宫颈炎有明显疗效；对阻断ＨＰＶ－１６的母婴传播有明显作用；
Objective:To demonstrate the chronic cervicitis being related to papilomavirus type 16(HPV-16),herpes simplex virus type 1 (HSV-2) and cytomegalovirus (CMV) infections ,and to observe the clinical efficacy of a recombinant human interferon alpha 1b (rhIFN-α1b)in treatment of chronic cervicitis and blocking the perinatal transmission of HPV-16. Methods:Using DNA hybridization technique,virus isolation and PCR method from samples taken from erosive,normal cervices as well as cervices of pregnant women with 38th gestational weeks. Results: The chronic cervicitis was shown to be highly related to HPV-16,HSV-2 and CMV infections as demonstrated by comparision with virus detection rate from erosive and normal crvices ;after one course of treatment with rhIFN-α1b was also to effective on block the perinatal transmission of a HPV-16,the perinatal transmission rate dropped down from 80% to 29.4% after treatment.Astragalus membranaceus(AM),a Chinese herbal durg,showed a synergic effect on interferon therapy. Conclusion: 1) Chronic cervicitis is closely related to viral infections; 2) A recombinant human interferon alpha 1b is very effective to treat the chronic cervicitis and block the perinatal transmission of HPV-16; 3)AM has a synergism to interferon therapy.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1998年第6期65-66,共2页
Journal of Jinan University(Natural Science & Medicine Edition)
关键词
慢性
宫颈炎
干扰素
乳头瘤病毒
治疗
chronic cervicitis
interferon α1b
HPV-16
perinatal transmission
