摘要
背景:器官移植后的患者常规使用地塞米松、他克莫司、环孢素A和麦考酚酸等临床常用的免疫抑制剂,其作用机制各不相同。有关不同免疫抑制剂对单核细胞趋化蛋白1的作用尚未见报道。目的:观察不同免疫抑制剂对全血细胞分泌单核细胞趋化蛋白1的影响。方法:取健康志愿者全血与不同免疫抑制剂(他克莫司,环孢素A,地塞米松,麦考酚酸,每种免疫抑制剂又分为低、中、高3个质量浓度)作用6h后,用佛波酯联合离子霉素刺激6h,比较不同免疫抑制剂组及免疫抑制剂联合使用单核细胞趋化蛋白1的水平。以全血单独培养作为对照。结果与结论:麦考酚酸和他克莫司不能有效抑制单核细胞趋化蛋白1的分泌;1,10mg/L地塞米松、0.05,1.25mg/L环孢素A均能够显著抑制单核细胞趋化蛋白1的分泌。因此,地塞米松和环孢素A可能通过抑制单核细胞趋化蛋白1的水平抑制单核巨噬细胞在免疫系统中发挥作用的。联合使用5μg/L他克莫司、10mg/L麦考酚酸和1mg/L地塞米松或者联合使用0.25mg/L环孢素A、10mg/L麦考酚酸和1mg/L地塞米松都可以有效抑制单核细胞趋化蛋白1的分泌,而上述免疫抑制剂单用时,仅地塞米松能有效抑制单核细胞趋化蛋白1的分泌。
BACKGROUND：In the field of organ transplantation,patients often take immunosuppressants after organ transplantation,such as CsA,FK506,DEX and MPA.However,their mechanisms of immunosuppression are different.The effect of immunosuppressive drugs on monocyte chemoattractant protein-1（MCP-1）remains poorly understood.OBJECTIVE：To investigate the effects of different immunosuppressants on the secretions of MCP-1 in whole blood.METHODS：The whole blood of healthy volunteers was mixed with different immunosuppressants for 6 hours,such as CsA,FK506,DEX and MPA,which included low,middle and high concentrations,followed by PMA and IONO stimulation for 6 hours.MCP-1 levels in whole blood samples were compared.The whole blood cultured alone served as control.RESULTS AND CONCLUSION：MCP-1 secretion was inhibited by DEX（1,10 mg/L）and CsA（0.25,1.25 mg/L）.However,FK and MPA exhibited no such effect.Therefore,DEX and CsA may inhibit the function of monocytes and macrophages in immune system by diminishing the secretion of MCP-1.The combination of FK（5 μg/L）,MPA（10 mg/L）and DEX（1mg/L）or CsA（0.25 mg/L）,MPA（10 mg/L）and DEX（1 mg/L）can inhibit the secretion of MCP-1,but only DEX among all the immunosuppressants mentioned above exhibited significant effect on inhibiting the secretion of MCP-1 when using alone.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第18期3314-3317,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
