摘要
目的观察重组合异种骨（ＲＢＸ）的成骨活性及其量效关系。方法采用ＲＢＸ移植建立ＢＡＬＢ／Ｃ小鼠股后肌袋模型，术后定期对移植组织进行放射学、病理学及碱性磷酸酶（ＡＬＰ）活性检查。结果（１）含不同比例的牛骨形态发生蛋白（ｂＢＭＰ）的ＲＢＸ组，成骨活性与ｂＢＭＰ含量呈正相关（ｒ＝０７２０４，Ｐ＜００１），存在着剂量依赖关系；（２）ＲＢＸⅠ组成骨良好，同样含量的纯ｂＢＭＰ组也出现可见的成骨效应，但最终未能成骨；（３）ＡＬＰ活性以术后７天最高，４２天时仍较明显。结论ＲＢＸ是高效诱导成骨材料，经处理的同源松质骨粒是良好的缓释载体。
Objective To observe osteoinductivity and the dose effect relationship with administration of reconstituted bone xenograft(RBX). Method A posterior thigh muscle pouch model with RBX transplantation was established in BALB/C mice.Samples were treated at regular postoperative intervals for radiologic,histomorphologic and ALP examinations. Result (1)In the groups that had been implanted with RBX, the osteoinductivity was positively correlated with the bBMP contents in RBX ( r =0 7204, P <0 01),showing a dose dependent relationship; (2) satisfactory osteogenesis was noted in the RBXⅠ group,whereas no substantial bone formation was seen in the group implanted with bBMP alone apart from some evidence of osteogenic effect; (3) the alkaline phosphatase activity attained its peak on the 7th day postoperatively and kept still higher to the 42nd day. Conclusion RBX is a highly osteoinductive grafting material with the treated homologous cancellous bone being a good slow delivery carrier which can enhance the osteoinductive capacity of bBMP.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1998年第10期627-629,I121,共4页
Chinese Journal of Surgery
关键词
骨骼移植
异种移植
RBX
成骨活性
量效关系
Bone transplantation Transplantation,heterologous Dose response relationship,drug Reconstituted bone xenograft
