摘要
目的探讨抗应激复合营养素对急性重症血管源性脑水肿(vasogenic brain edema,VBE)应激下超氧化物歧化酶(SOD)、丙二醛(MDA)和脑组织含水量的影响,为提高急性重症脑卒中患者抗应激能力提供实验依据。方法将96只SD雄性大鼠随机分成3组各32只:(1)对照组,予去离子水灌胃1周后腹腔注射10%苯肾上腺素造成急性VBE模型,并观察造模后1h、2h、6h和12hSOD、MDA和脑组织含水量的变化情况;(2)实验1组,予维生素C、维生素E、L-精氨酸和谷氨酰胺组成的复合营养素灌胃1周,其造模方法、采血时间点及观察指标同对照组;(3)实验2组,予维生素C、维生素E、L-精氨酸、谷氨酰胺和氯化镁组成的复合营养素灌胃1周,其造模方法、采血时间点及观察指标同对照组。结果对照组、实验1组和实验2组之间血清SOD和MDA含量变化差异有统计学意义(P<0.05),且实验2组优于实验1组;3组对应时间点之间脑组织含水量差异有统计学意义(P<0.05),且实验2组、实验1组较对照组脑水肿程度较轻。结论早期给予肠内抗应激复合营养素能有效降低机体应激反应,保护器官,减少并发症,维生素C、维生素E、L-精氨酸、谷氨酰胺和氯化镁组成的复合营养素组方具有更强的抗应激效果。
Objective To explore the influence of the compound nutrients on superoxide dismutase （SOD）, malondidehyde （MDA） and brain water content under the vasogenic brain edema （VBE） stressed condition, so as to provide the basis for improving acute stroke patients＇ anti-stress capability. Methods 96 SD male rats were randomly divided into three groups： （1） control group, fed deionized water for a week, intraperitoneal injection of 10% of the phenylephrine caused by acute VBE model, and then the changes of the brain water content, SOD, and MDA after modeling 1 h, 2 h, 6 h and 12 h were observed; （2） experimental group 1, who were offered gastric perfusion of vitamin C, vitamin E, L-arginine and glutamine composition of complex nutrients for a week, with the modeling methods, blood collection time points and observed targets the same as the control group; （3） experimental group 2, who were offered the gastric perfusion of vitamin C, vitamin E, L-arginine, glutamine, and magnesium chloride composition complex nutrients for a week, with the modeling methods, blood collection time points and observed indicators the same as the control group. Results The changes in SOD and MDA levels between the three groups were significantly different （P〈0.05）, with the experimental group 2 better than experiment group 1; there also existed significantly differences in brain water content at corresponding time points between the three groups （P〈0.05）, with the edema of experimental group 1 and group 2 less severe. Conclusion Early administration of anti-stress response compound enteral nutrients can effectively redui^e the body＇s stress response, protect organs, reduce complications, bearing testimony to the sound anti-stress effect.
出处
《护理学报》
2010年第7期22-26,共5页
Journal of Nursing（China）
基金
广东省自然科学基金(07005163)
关键词
急性脑水肿
应激反应
营养素
acute cerebral edema
stress response
nutrient
