摘要
目的观察芪薏益肝煎对H22荷瘤小鼠的抑瘤作用,并探讨其作用机制。方法小鼠右腋下皮下接种传代7天的腹水型H22肝癌细胞,建立实体瘤模型。将30只小鼠分为3组,对照组、中药组和5-FU组,每组10只;用药15天后取瘤称重、计算抑瘤率,采用免疫组化技术检测survivin和caspase-3的表达。结果芪薏益肝煎对小鼠肝癌细胞的生长抑制率为37.3%,5-FU组为50.5%。survivin及caspase-3在三组中均呈阳性表达,中药组和5-FU组survivin表达减弱,与对照组比较差异有统计学意义(P<0.05、P<0.01)。caspase-3表达增强(P<0.01)。结论芪薏益肝煎对肝癌细胞的增殖有明显的抑制作用,其作用机制与survivin及caspase-3表达有关。
Objective To investigate the antitumor effect of Chinese medicine Qiyiyiganjian on ascites hepatoma H22 in mice.Methods After H22 hepatoma cells were inoculated into right axilla,30 mice were randomly divided into 3 groups with 10 in each：control group receiving normal saline,treatment group treated with Qiyiyiganjian and 5-FU group treated with 5-FU.Inhibition rate was calculated 15 days after treatment;survivin and caspase-3 were examined by immunohistochemistry method in tumor tissue samples.Results The inhibition rates for Qiyiyiganjian and 5-FU groups were 37.3% and 50.5%,respectively.Compared with control group,the expressions of survivin in Qiyiyiganjian and 5-FU groups were decreased（P〈0.05 and P〈0.01）,and the expression of caspase-3 was increased（P〈0.01）.Conclusion Chinese medicine Qiyiyiganjian can inhibit the growth of ascites hepatoma cells in mice,which is associated with the down-regulation of survivin and up-regulation of caspase-3 in tumor cells.
出处
《实用肿瘤杂志》
CAS
北大核心
2010年第2期167-170,共4页
Journal of Practical Oncology
