摘要
目的探讨纤溶酶原激活剂抑制物-1(plasminogen activator inhibitor-1,PAI-1)基因启动子区4G/5G多态性与特发性肺纤维化(IPF)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析,检测42例IPF患者和164例正常对照组人群PAI-1基因4G/5G多态性。结果PAI-1基因4G/4G、4G/5G、5G/5G基因型频率分布,IPF组分别为31.0%、50.0%、19.0%,对照组分别为17.1%、54.9%、28.0%;4G和5G等位基因频率,IPF组分别为0.560和0.440,对照组分别为0.445和0.555;4G/4G基因型频率IPF组显著高于对照组(P<0.05)。与4G/5G和5G/5G基因型比较,携带4G/4G型个体发生IPF的风险增加2.18倍,95%CI:1.02～4.68(P<0.05)。结论PAI-1基因4G/5G多态性与IPF的发病相关,纯合子4G/4G基因型可能是IPF发病的重要危险因素之一。
Objective：To investigate the relationship between polymorphism of plasminogen activator inhibitor-1（PAI-1）gene 4G/5G and idopathic pulmonary fibrosis（IPF） in Weifang population.Methods：In this case-control study,the PAI-1 gene 4G/5G polymorphism was determined by PCR-RFLP in 42 IPF patients and 164 normal controls from the rural area of Weifang. Results：The frequencies of PAI-1 gene 4G/4G,4G/5G and 5G/5G genotypes were 31.0%,50.0% and 19.0%,respectively in IPF group,and 17.1%,54.9% and 28.0%,respectively in control group.The frequenceies of 4G and 5G alleles were 0.560 and 0.440 in IPF group,and 0.445 and 0.555 in control group.There was statistically significont difference in the frequency of 4G/4G genotype for PAI-1 gene in two groups （P0.05）.As compared with 4G/5G and 5G/5G genotypes,the individual with 4G/4G genotype was 2.18 times susceptible to IPF than the normal controls,95%CI：1.02～4.68 （P0.05）.Conclusion：PAI-1 gene 4G/5G polymorphism is associated with IPF.The 4G/4G genotype is one of susceptible risk factors of IPF.
出处
《中国优生与遗传杂志》
2010年第4期24-25,共2页
Chinese Journal of Birth Health & Heredity
