摘要
目的探讨β-淀粉样蛋白1-42诱导的模型大鼠海马区乙酰胆碱酯酶(AChE)和胆碱乙酰化酶(choline acetyltransferase,ChAT)的表达及其七福饮的干预作用。方法采用海马立体定向注射凝聚态β-淀粉样蛋白1-42制备老年性痴呆大鼠实验动物模型。七福饮高、中、低3个剂量组灌胃,进行药物干预,给药4周后,处死大鼠,取海马组织,应用免疫组化SP方法及病理图像定量分析AChE、ChAT的表达。结果与假手术对照组比较,模型组AChE表达增高(P<0.05),而ChAT表达减少(P<0.05);与模型组比较,盐酸多奈哌齐对照组、七福饮各剂量AChE表达均显著降低(P<0.05),而ChAT表达均显著增高(P<0.05)。结论七福饮可能通过下调大鼠海马组织中的AchE表达和上调ChAT表达,进而发挥抗老年性痴呆的作用。
Objective To investigate the effect of Qifu-yin on the expression of Acetylcholinesterase （AchE） and choline acetyltransferase （Chat） in experimental AD rats induced by Aβ1-42. Methods SD rats were equally randomly divided into blank controls group,sham controls group,model control group,donepezil control group and Qifu-yin groups （2.15 mg·kg^-1·d^-1,4.3 mg·kg^-1·d^-1,8.6 mg·kg^-1·d^-1）. The AD model was established by amyloid-β1-40 sterotaxis injection in the bilateral Hippocampus. After treatment for 4 weeks,rats were sacrificed and the hippocampus was harvested,the expression levels of AchE and Chat were measured by immunohistochemical SP techniques,and the results were assessed by an image analysis system. Results Compared with the sham control group,the expression of AChE protein was up-regulation significantly in model group （P〈0.05）,while Chat was down-regulation （P〈0.05）. Expression of AChE was down-regulation,and Chat was up-regulation in donepezil and Qifu-yin groups （2.15 mg·kg^-1·d^-1,4.3 mg·kg^-1·d^-1,8.6 mg·kg^-1·d^-1） compared with the model control group （P〈0.05）. Conclusions Qifu-yin may be a potential candidate for development as a therapeutic agent to down-regulate AChE and up-regulate.
出处
《齐齐哈尔医学院学报》
2010年第4期505-507,共3页
Journal of Qiqihar Medical University
基金
黑龙江省教育厅资助面上项目(编号:11531435)
关键词
七福饮
老年性痴呆
海马
乙酰胆碱酯酶
胆碱乙酰转移酶
Qifu-yin Alzheime＇s disease Hippocampus Acetylcholinesterase Choline acetyltransferase
