摘要
为阐明骨舌鱼科鱼类的遗传结构和进化关系,测定了美丽硬仆骨舌鱼Scleropages formosus的3个品种金龙(Gold arowana)、红龙(Red arowana)、青龙(Green arowana)细胞色素b(cyt b)基因全序列(1 141 bp),结合来自GenBank中珍珠龙Scleropages leichardti、星点珍珠龙S.jardini、非洲龙Heterotis niloticus、黑龙Osteoglossum ferrerirai、银龙O.bicirrhosum、海象Arapaima gigas的全序列,用邻接法(NJ)和最大简约法(MP)构建分子系统树,初步分析了骨舌鱼科鱼类的系统发育关系.NJ树和MP树均一致表明,骨舌鱼类为单系类群,分为4支,支持骨舌鱼科下设4个属(坚体鱼属Scleropages、异耳鱼属Heterotis、骨舌鱼属Osteoglossum、巨骨舌鱼属Arapaima)阶元的分类系统,4个属的系统关系与其形态、生态特征表型进化和地理分布较为一致.而金龙、红龙和青龙的mtDNA Cytb的碱基差异小于1%,说明这3个龙鱼品种还在同一种的水平上.
The present study aimed to clarify the genetic structure and evolutionary relationships within Osteoglossidae,using complete mitoehondrial cytochrome b（Cyt b） gene sequences （1 141 bp） of three breeds of Scleropages formosus ： Gold arowana, Red arowana and Green arowana, together with relevant sequences of Scleropages leichardti, S. jardini ,Heterotis niloticus, Osteoglossum ferrerirai, O. bicirrhosum ,Arapaima gigas obtained from GenBank. The phylogenetic trees constructed by two methods （ NJ and MP） supported the same topology, and molecular phylogenetic relationships of fishes of the Osteoglossidae were constructed. Both of NJ tree and MP tree showed that fishes of the Osteoglossidae were a monophyletic group and classified Osteoglossidae into four genera, namely Scleropages, Heterotis, Osteoglossum,Arapaima, which were perfectly consistent with their morphological, ecological characteristics, evolution and geographic distribution pattern. Sequence variation of less than 1% in mtDNA Cyt b gene among three breeds of S. formosus suggested that they might represent the same species.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2010年第2期100-103,共4页
Journal of South China Agricultural University
基金
农业部"948"项目(2009-Z13)
农业部淡水鱼类遗传育种和养殖生物学重点开放实验室基金(BZ2009-10)
中国水产科学研究院科研基金项目(2003-3-5)
广州市荔湾区科技计划(20082109029)
