摘要
对已公布在NCBI数据库中的巨魾(Bagarius yarrelli)全基因组测序结果,使用MISA软件对巨魾全基因组中的微卫星进行筛选并分析其数量与分布特征.在巨魾基因组570806968 bp序列中,共筛选出360235个完整型微卫星,其长度为6998449 bp,占基因序列总长度的1.23%.在6种完整型微卫星中,微卫星数量最多的是单碱基类型,约占总数的44.65%,其余碱基类型数量排序为二碱基(43.29%)、三碱基(6.12%)、四碱基(4.80%)、五碱基(1.02%)和六碱基(0.11%).基因组中数量最多的前10种微卫星类别分别为:A、AC、AG、AT、AAT、C、ATAG、AAAT、ACT和ATC.
The study uses the published genome-wide sequencing results of the Bagarius yarrelli on NCBI to screen and analyze the number and distribution of the microsatellites of the whole genome using MISA software.In the 570806968 bp longsequence of the Bagarius yarrelli genome,360235 perfect microsatellites are screened with a length of 6998449 bp,accounting for 1.23%of the total length of the genome sequence.Among the six types of microsatellites,mononucleotide is the most,accounting for 44.65%of the total,the other bases are dinucleotide(43.29%),trinucleotide(6.12%),tetranucle-otide(4.80%),pentanucleotide(1.02%)and hexanucleotide(0.11%).The top 10 microsatellite copy types in the genome are:A,AC,AG,AT,AAT,C,ATAG,AAAT,ACT and ATC.
作者
杨汶珊
唐荣叶
苏孟园
徐杰杰
王涛
尹绍武
Yang Wenshan;Tang Rongye;Su Mengyuan;Xu Jiejie;Wang Tao;Yin Shaowu(School of Marine Science and Engineering,Nanjing Normal University,Nanjing 210023,China;Jiangsu Province Engineering Research Center for Aquati Animals Breeding and Green Efficient Aquacultural Technology,Nanjing 210023,China)
出处
《南京师范大学学报（工程技术版）》
CAS
2021年第3期62-68,共7页
Journal of Nanjing Normal University(Engineering and Technology Edition)
基金
江苏省农业重大新品种创制项目(PZCZ201742)
江苏省重点研发计划(现代农业)重点项目(BE2017377)
江苏省农业科技自主创新资金项目(CX(19)2034)
南京师范大学大学生创新创业训练计划项目.
关键词
巨魾
全基因组
微卫星
分布特征
Bagarius yarrelli
genome
microsatellites
distribution characteristics
