摘要
以一个月月龄银杏(Ginkgo biloba)无菌幼苗各部位茎段为外植体,研究其顶芽、带腋芽的中段、带子叶的下段腋芽萌发和不定芽的分化情况。结果表明:①银杏顶芽段(上段)在以改良MS、N6、DCR为基本培养基,附加0.1mg/LNAA和0.5mg/L6-BA的培养基上顶芽生长较其他培养基好,抽出的芽苗长得高,最高达2.98cm,未见有不定芽的分化;②中段外植体在四种基本培养基(MS、DCR、N6、改良MS)中附加0.1mg/LNAA和0.5mg/L6-BA和0.25%AC时,都能诱导出腋芽和不定芽,4种培养基之间芽诱导率无明显差异,以N6培养基的诱导效果最好,平均每个外植体产生1.56个芽;③带有子叶下段的外植体培养后,能诱导出2～3个子叶腋芽和新的不定芽,在改良MS+0.1mg/LNAA+0.5mg/L6-BA、N6+0.1mg/LNAA+0.5mg/L6-BA培养基上两个腋芽的诱导率均达80%以上。表明,不同部位的银杏茎段在芽的诱导上存在位置效应,可利用不同部位进行银杏的定向快速繁殖。
Experiment was conducted by taking stem segments including terminal buds, segment with axillary buds, segments with cotyledon of 1-month seedling as explants for studying germination and differentiation of adventitious buds of Ginkgo biloba. The result demonstrated that growth of terminal bud segments （upper section） was better in the medium of modified MS, N6, DCR supplemented with 0.1 mg/L NAA and 0.5 mg/L 6-BA than in other media, the highest shoot was up to 2.98 cm. But the adventitious buds were not observed. The explants of segments with axillary buds could induce axillary buds and adventitious buds in four media （MS, DCR, N6, modified MS） supplemented with 0.1 mg/L NA, A and 0.5 mg/L 6-BA and 0.25%AC, and the induction rate had no significant difference among the four media, but N6 had better effect with 1.56 bud per explant. Segments with cotyledons could be induced with 2 - 3 axillary buds and adventitious buds. The two axillary buds induction rate was more than 80% in the media of modified MS ＋ 0.1 mg/L NAA ＋ 0.5 mg/L 6-BA and N6 ＋ 0.1 mg/L NAA ＋ 0.5 mg/L 6-BA. The results indicated that different stem segments of G. biloba had different induction effect.
出处
《浙江林业科技》
北大核心
2010年第1期28-31,共4页
Journal of Zhejiang Forestry Science and Technology
基金
江苏省高校自然科学研究计划项目(07KJB220048)
关键词
银杏茎段
组织培养
位置效应
stem of Ginkgo biloba
tissue culture
position effect
