摘要
利用南京11×Koshihikari RIL群体在南京两年的种植结果,检测与稻米淀粉RVA谱特征值峰值黏度、最低黏度、最终黏度、崩解值、消减值、峰值时间和糊化温度相关加性和上位性效应QTL,并分析其表达稳定性。结果表明,7个稻米淀粉RVA谱特征值在两年中均呈连续分布,且存在超亲遗传现象。共检测到8个具有加性效应的QTL与稻米6项淀粉RVA谱特征值有关,两年中能重复出现的QTL有6个,即qTPV-6、qFPV-6、qBDV-6、qSBV-6、qPKT-6和qPT-6,分别控制最低黏度、最终黏度、崩解值、消减值、峰值时间和糊化温度,两年的平均贡献率分别为46.4%、60.3%、31.1%、71.9%、38.5%和12.4%,而qSBV-8和qPT-5仅在1年中被检测到,环境稳定性差。此外,还检测到5对影响稻米淀粉RVA谱特征值的QTL具有上位性效应,互作既发生在相同染色体上也发生在不同染色体之间,但贡献率较小,且受环境的影响较小。控制稻米淀粉RVA谱特征值的稳定、主效QTL,可为Koshihikari中稻米优良蒸煮食味品质相关基因的育种利用提供基础。
Additive QTLs,epistatic QTLs and expression stability of QTLs for rice starch RVA profile properties were analyzed using Nanjing 11/Koshihikari RIL population in Nanjing in 2006 and 2007.The studied RVA profile properties involved peak viscosity （PKV）,trough paste viscosity （TPV）,final paste viscosity （FPV）,breakdown viscosity （BDV）,setback viscosity （SBV）,peak time （PKT） and pasting temperature （PT）.The normal distribution and transgressive segregation were observed in the seven RVA profile characteristics of the RIL population in two years.Eight additive QTLs were detected for 6 RVA profile characteristics,and 6 QTLs with main effect （qTPV-6,qFPV-6,qBDV-6,qSBV-6,qPKT-6 and qPT-6） were repeatedly detected in two years,which controlled trough paste viscosity,final paste viscosity,breakdown viscosity,setback viscosity,peak time and pasting temperature,with average contribution rates of 46.4%,60.3%,31.1%,71.9%,38.5% and 12.4%,respectively.qSBV-8 and qPT-5 were detected in one environment,indicating that these two QTLs were easy to be influenced by environments.Otherwise,five QTLs with epistatic effects with little contribution rate were detected in the same chromosome or between different chromosomes,which were little affected by environments.The QTL analysis for RVA profile properties in rice could lay the foundation for utilization of superior cooking and eating quality related genes in Koshihikari in molecular breeding.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2010年第2期137-144,共8页
Chinese Journal of Rice Science
基金
国家973计划前期预研资助项目(2008CB117004)
国家863计划资助项目(2006AA10Z1A5
2009AA101101)
国家科技支撑计划资助项目(2008BAD97B02)
江苏省科技支撑计划资助项目(BE2008354
BE2009301-3)
关键词
稻米
淀粉黏滞性谱
数量性状基因座
加性效应
上位性
表达稳定性
rice
starch viscosity profile
quantitative trait locus
additive effect
epistatic effect
expression stability
