摘要
目的:探讨高良姜配方颗粒质量控制方法并为其质量控制提供参考。方法:采用薄层色谱法对不同批次高良姜配方颗粒进行定性鉴别;采用HPLC法测定其中高良姜素和山奈素的含量。phenomenex Gemini C18(4.6 mm×250 mm,5μm)色谱柱;流动相甲醇-0.4%磷酸(58∶42);检测波长360 nm;流速1.0 mL.min-1;柱温30℃。结果:薄层色谱斑点分离较好,斑点清晰;高良姜素在0.104～1.044μg呈线性关系,r=0.999 3,平均回收率98.05%,RSD 1.96%(n=6);山奈素在(2.2～21.6)ng内呈线性关系,r=0.999 2,平均回收率99.71%,RSD 1.86%(n=6)。结论:该方法简便快速,准确可靠,专属性强,可作为高良姜配方颗粒的质量控制方法。
Objective：To explore the quality control standard of Alpinia officiarum Formula Granules and to provide reference for it.Method： TLC was used to identify Alpinia officiarum Formula Granules in different bat-ches.HPLC was used for the determination of galangin and kaempferol.The chromatographic column was phenome-nex Gemini C18（4.6 mm × 250 mm,5 μm）;the mobile phases were methanol-0.4% phosphoric acid（58∶ 42）;the detection wavelength was set at 360 nm;the flow rate was at 1.0 mL.min-1 and the column temperature was at 30 ℃ respectively.Result： The TLC spots were clear and well divided.Galangin was linear in the range of（0.104 ～ 1.044）μg,r = 0.999 3,and the average recovery was 98.05% with RSD = 1.96%（n = 6）;Kaempferol was also linear in the range of（2.2 ～ 21.6）ng,r = 0.999 2,and the average recovery was 99.71% with RSD 1.86%（n = 6）.Conclusion： This method can be used as a quality control standards for Alpinia officiarum Formula Granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第4期21-23,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部"十一五"科技支撑项目(2006BAI06A14-06)
