摘要
目的观察廊小板源性生长因子-D（PDGF—D）在膀胱移行细胞癌（BTCC）中的表达，探讨PDGF—D与膀胱移行细胞癌的发生发展之间的关系及其临床意义。方法分别采用实时定量逆转录一聚合酶链反应（real—timeRT—PCR）法和蛋白免疫印迹（Westernblot）法检测62例膀胱移行细胞癌组织、10例癌旁正常膀胱组织中PDGF—DmRNA和蛋白的表达，并分析其与临床病理之间的关系。结果PDGF—DmRNA在BTCC中的表达量是正常对照组的1．89倍，PDGF—D蛋白在癌组织中的表达明显高于正常膀胱组织（相对吸光度比值：正常组0．231±0．041，癌症组0．689±0．083，P〈0．05）。而且随着肿瘤病理分级的升高及淋巴结转移的出现，PDGF—DmRNA和蛋白的表达水平均逐渐升高，各组间差异有统计学意义（P〈0．05）。但不同分期的癌组织中PDGF—DmRNA和蛋白的表达水平的差异无统计学意义（P〉0．05）。结论PDGF—D表达增强与膀胱癌的发生及分化、转移密切相关，可能在膀胱癌的发生发展中发挥重要作用。
Objective To investigate the expression of PDGF-D in bladder transitional cell carcinoma （BTCC） , and its clinical significance in tumorigenesis and progression of BTCC. Methods By using real-time reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting, the expression levels of PDGF-D mRNA and protein were detected in 62 cases of BTCC tissues, and 10 cases of the normal bladder tissues which were closely adjacent to the carcinoma. The relationship between PDGF-D expression and the clinicopathological features was analyzed. Results The expression levels of PDGF-D mRNA in BTCC were 1.89 times as much as those in the normal control group. PDGF-D protein expressed in tumor tissues was significantly higher than in normal bladder tissues （absorbance ratio in the normal bladder tissues was 0.231 ±0. 041, and that in the tumor tissues was 0. 689 ±0. 083, P 〈 0.05 ）. Moreover, with the grades going up and appearance of lymph node metastasis, the expression levels of PDGF-D mRNA and protein were increased gradually （P 〈 0. 05 ）. But in different clinical stages ,the expression levels of PDGF-D mRNA and protein had no significant difference （ P 〉 0.05 ）. Conclusion The overexpression of PDGF-D is closely correlated with the occurrence, differentiation and lymphatic metastasis of BTCC, and may play an important role in carcinogenesis and progression of bladder cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第3期287-289,共3页
Chinese Journal of Experimental Surgery
