摘要
对98份普通野生稻、亚洲栽培稻及稻进行了核糖体RNA基因间间隔区的限制性片段长度多态性（RFLP）分析。共发现30种长度变异类型，组成45种表现型。广西普通野生稻的rDNA间隔序列长度多样性最丰富，24份材料中检测到25种长度变异类型，共组成22种表现型。rDNA间隔区长度变异类型的地理分布与纬度相关，高纬度来源的普通野生稻的rDNA间隔区长度较短，与粳稻的长度变异类型相似；低纬度来源的材料rDNA间隔长度趋长，与籼稻的长度变异类型相似；稻的长度变异类型与粳稻相似。提出了华南是野生稻变异中心的观点，并指出对我国野生稻资源进行保护的重要性。
A sample of 98 accessions of the common wild rice (Oryza rufipogon), Asiancultivated rice and Ludao, mostly from China, were assayed for ribosomal DNApolymorphisms. A total of 30 spacer length variants (slys), which formed 45 rDNAphenotypes, were detected. Wild rice from Guangxi is the richest in diversity; 25 slvswere detected among 24 accessions. The distribution of rDNA spacer length variantswere relaied to latitudes where the samples were collectod. Samples from higherlatitudes showed shorter slys, while those from lower latitudes showed longer ones.Such geographical distributions seem to correspond well with indica-japonicadifferentiation. Ludao is similar to japonica rice in rDNA slvs. The data suggestedthat Southern China is likely to be one of the centers of genetic diversity of wild rice.The results also indicated the importance of conservation and utilization of wild ricegermplasm in China.
基金
国家自然科学基金!38970440
关键词
核糖体RNA基因
遗传多样性
野生稻
Oryza rufipogon, rDNA, Intergenic spacer, Genetic diversity
