摘要
目的探讨KISS-1基因与膀胱癌转移的相关性,及其稳定表达对膀胱癌细胞细胞侵袭能力的影响。方法应用荧光定量PCR方法检测原发未转移膀胱癌和原发伴转移膀胱癌组织中KISS-1 mRNA的表达。构建真核表达载体pIRES2-KISS1,转染膀胱癌T24细胞系并筛选单克隆稳定转染细胞株,检测转染前后细胞的侵袭能力变化。结果原发伴转移膀胱癌组织中的KISS-1 mRNA表达较原发未转移膀胱癌中明显下降,具有统计学差异(P<0.05)。单克隆稳定转染细胞系中KISS-1蛋白明显增加,细胞侵袭能力也随之明显降低(P<0.05)。结论KISS-1基因与膀胱癌的转移相关,其表达增高能够抑制T24细胞的侵袭能力。
Objective To explore the relationship between KISS-1 gene and metastasis of bladder carcinoma,and to study the effect of the stable expression of KISS-1 gene on the invasion of bladder carcinoma cell line T24. Methods Fluorescent quantitative PCR was used to detect the expression of KISS-1 mRNA in primary bladder carcinoma without metastasis and primary bladder carcinoma with metastasis. Recombinant vector pIRES2-KISS-1 was constructed and transfected into T24 cells. Single clone of stably transfected cells was screened,and the changes in the invasive ability of T24 cells was detected after transfection. Results The expression level of KISS-1 mRNA in primary bladder carcinoma with metastasis was significantly lower than that in primary bladder carcinoma without metastasis（P 0.05）. The expression of KISS-1 protein in the single clone of stably transfected cells increased significantly,and the invasive ability significantly decreased（P 0.05）. Conclusion KISS-1 gene is correlated with the metastasis of bladder carcinoma,and the up-regulated expression of KISS-1 gene can inhibite the invasiveness of T24 cell line.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第3期168-170,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30901480
30700980)
