摘要
取丽格海棠叶片作外植体,通过试验,分析不同激素配比对芽的诱导、增殖及生根的影响,筛选出丽格海棠叶片组织培养的适宜培养基及试管苗最佳移栽基质。结果表明:其最佳诱导培养基为MS+6-BA 1.5 mg/L+NAA0.8 mg/L,不定芽诱导率达100%;最佳继代培养基是MS+6-BA 1.0 mg/L+NAA 0.5 mg/L,增殖倍数达6.5倍;最佳生根培养基是1/2 MS+NAA 0.4 mg/L,生根率达100%;试管苗最佳移栽基质为用1/2MS大量元素浇透的草炭,移栽成活率为85%。
Using leaves of Rieger Begonia as explant, the optimal culture medium was screened out by analyzing the effects of various hormone proportions on bud induction and proliferation and the effect of hormone concentration on rooting. The results showed that： the optimum medium for induction is MS＋6-BA 1.5 mg/L＋ NAA 0.8 mg/L ; induction rate of adventitious bud reaches 100%; The optimum subculture medium is MS＋ 6-BA 1.0 mg/L＋NAA 0.5 mg/L, and proliferation times 6.5; The best rooting medium is 1/2MS＋NAA 0.4 mg/L with 100% of rooting rate; The optimal transplanting medium for tube seedling is the peat irrigated through with half of MS abundant element with transplanting survival rate of 85%.
出处
《山西林业科技》
2010年第1期14-15,21,共3页
Shanxi Forestry Science and Technology
关键词
丽格海棠
叶片
组织培养
Rieger Begonia
leaves
tissue culture
