摘要
用高蔗糖饲料喂养雄性ＳＤ大鼠，诱发高胰岛素血症及胰岛素抵抗。实验发现第２周时高糖组血糖明显低于对照组，４周后与对照组相比无明显区别。高糖组血清胰岛素水平从第２周开始升高，４周后升高更为明显（Ｐ＜００１）。饲养２０周时取肝，制备肝细胞膜可溶性胰岛素受体，经胰岛素受体放射配体法测定，发现高糖组与对照组间受体亲和力无明显差别，但高糖组的受体最大特异结合与受体浓度低于对照组（Ｐ＜０．０５），说明高蔗糖膳食可引起雄性ＳＤ大鼠高胰岛素血症。
We fed male SD rats with high sucrose diet to induce hyperinsulinemia and insulin resistance. The serum glucose concentration of high sucrose diet (HSD) group was lower than that of the control in the 2nd week, serum insulin level of HSD group incresed. Four weeks later, insulin level was getting hihger and higher than that of the control one (P<0.01). We observed that HSD could induce hyperinsulinemia and IR, but no significant change at serum glucose level. The biggest special insulin binding and the concentration of solubilized insulin receptor from rats liver in HSD group were lower than that of the control group (P<0.05), and correlated to serum insulin level, we demonstrated that high sucrose diet can induce hyperinsulinemia and the changes of the kinetics of insulin receptor binding can aggravate insulin resistance in male SD rats.
出处
《实验动物科学与管理》
1998年第4期1-4,共4页
Laboratory Animal Science & Administration
基金
天津自然科学基金
关键词
高蔗糖饲料
高胰岛素血症
胰岛素受体
胰岛素抵抗
high sucrose diet\ hyperinsulinemia\ insulin receptor \ insulin resistance
