摘要
目的：观察吗啡条件性位置偏爱（CPP）重现大鼠海马内脑源性神经营养因子（BDNF）及即早基因c．fos的表达，探讨BDNF和c-fos对吗啡成瘾记忆的影响。方法：雄性SD大鼠36只，随机分为吗啡CPP激发组（MR组）、吗啡CPP消退组（ME组）及生理盐水对照组（Ns组），每组12只。实验第1～7天，MR组、ME组采用CPP实验，建立大鼠吗啡精神依赖动物模型，实验第8天进行训练后CPP测试，给予7d消退期，实验第16天进行消退后测试，实验第17天对MR组大鼠进行环境激发，第18天进行激发后CPP测试。记录CPP实验过程中15min内伴药箱停留时间；激发后CPP测试完毕后，应用免疫组化方法测定大鼠海马内BDNF和c—fos的表达。结果：3组不同时间点大鼠伴药箱停留时间差异有统计学意义（FH目=5．76，P=0．007；F组别=4．07，P=0．028）。MR组大鼠海马中BDNF、c—fos的阳性细胞数及平均光密度均高于NS组和ME组（P均〈0．01）。结论：BDNF、c-fos可能通过成瘾记忆的形成，参与环境激发的大鼠吗啡CPP重现。
Aim： To observe the expression of brain-drived neurophic factor（BDNF） and c-los in hippocampus of rats with morphine conditioned place preference（CPP） after environment induced reinstatement and to evaluate the influ- ence of BDNF and e-los on addiction memory. Methocls：A total of 36 rat were randomly allocated into 3 groups （normal saline control group, extinction group, and reinstatement group, 12 in each group）. Rats in extinction group and reinstate- ment group were to establish the CPP model of morphine. Reinstatement group was induced by environment after extinc- tion of CPP. The residence time in white box within 15 rain was recorded, and the expression of BDNF and c-fos in hip- pocampus of rats were estimated by immunohistoehemistry. ReSults ： There were significant differences in the residence time among the 3 groups at different time point（F,~e =5.76,P=O.007;Fsroup =4.07,P=0.028）. The mean optical density and the number of positive cells of BDNF and e-fos in hippoeampus significantly increased in reinstatement group compared with those of saline control group and extinction group（ P 〈0.01 ）. Conclusion ： BDNF and e-los may be relat- ed to the addiction memory and participate in the environment induced reinstatement to morphine CPP.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第2期198-201,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
贵州省优秀科技教育人才省长专项基金资助项目黔科教办[2008]04号
