摘要
采用流式细胞术，用单克隆抗体定量测定了２０例急性脑血栓形成患者周围血中白细胞ＣＤ１１ａ、ＣＤ１１ｂ的阳性表达率，并以２０例健康人作对照。结果：脑血栓形成组血液ＣＤ１１ａ在单核细胞、粒细胞、淋巴细胞的阳性表达率均明显高于对照组（Ｐ＜０．０５）；ＣＤ１１ｂ在粒细胞和单核细胞的阳性表达率也明显高于对照组（Ｐ＜０．０５），但它在淋巴细胞上的表达两组间则无显著性差异。由此提示脑血栓形成时白细胞ＣＤ１１ａ、ＣＤ１１ｂ表达上调，白细胞与内皮细胞的粘附明显增强，可能会加重缺血后迟发性神经元死亡的病理过程。
This study sought to understand the mechanism for the increased adhesion of leucocytes and endothelial cells in ischemic stroke. 20 patients with acute cerebral thrombosis and 20 healthy subjects as controls for expression of CD 11a and CD 11b (adhesion molecules on surface of leucocytes) were tested in vitro by flow cytometry (FCM) method. The results showed that compared with the control group, the patient group had significantly higher rates for expression of CD 11a on monocytes, granulocytes and lymphocytes ( P<0.05 ). The CD 11b expression in the patient group was positively elevated on monocytes and granulocytes ( P<0.05 ), but it was of lower positive rate on lymphocytes and no statistical difference was noted between the patient and control groups. These indicate that the expression of CD 11a and CD 11b on leucocytes increases in cerebral ischemic damage; thus adhesion of leucocytes and endothelial cells obviously increases. This change may aggravate post ischemic delayed neuronal death.
出处
《华西医科大学学报》
CSCD
1998年第4期415-417,共3页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
