摘要
本文建立了一种反相高效液相色谱-紫外光谱检测法,可同时定量检测维力波糖、米格列醇、维格列波糖和阿卡波糖这4种α-葡糖苷酶抑制剂。采用Prevail carbohydrate(250mm×4.6mm,5μm)色谱柱,以乙腈-磷酸盐缓冲液(pH8.0)进行梯度洗脱,在210nm下进行检测,结果维力波糖、米格列醇、维格列波糖和阿卡波糖的线性范围分别为58.2～932.0μg·mL-1(r=0.9999,n=5)、23.5～376.0μg·mL-1(r=0.9999,n=5)、0.128～2.050mg·mL-1(r=0.9999,n=5)、38.2～612.0μg·mL-1(r=0.9999,n=5);平均回收率分别为97.0%、103.6%、96.4%和100.4%(n=9)。与此同时,本文还建立高相液相色谱-质谱检测方法,用于这4种化合物的定性分析。以上两种方法简便快捷,结果准确可靠、重复性好,可用于可疑的糖尿病药品中非法添加α-葡糖苷酶抑制剂的定性定量检测。
An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography （Prevail carbohydrate column, 250 mm × 4.6 mm, 5 μm） with a gradient acetonitrile-phosphate buffer solution （pH 8.0） at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four α-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.
出处
《药学学报》
CAS
CSCD
北大核心
2010年第3期347-352,共6页
Acta Pharmaceutica Sinica
